PMID- 21519328
OWN - NLM
STAT- MEDLINE
DCOM- 20110923
LR  - 20230216
IS  - 1530-0307 (Electronic)
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 91
IP  - 8
DP  - 2011 Aug
TI  - Bone marrow cell-mediated production of transgenic chickens.
PG  - 1229-40
LID - 10.1038/labinvest.2011.53 [doi]
AB  - The transplantation of adult stem cells into recipients is a method used widely 
      in mammals to determine the fate of transferred cells, and for the production of 
      progenies. This study is the first report, to our knowledge, to demonstrate the 
      successful production of chickens using cells transdifferentiated from adult 
      chicken bone marrow cells (BMCs) transplanted into the testes. BMCs from the 
      enhanced green fluorescent protein (eGFP) transgenic (Tg) chickens were induced 
      via in vitro transdifferentiation to male germ cells and injected into the testes 
      of normal recipients. The multipotency of BMC was found with RT-PCR, 
      immunocytochemistry, and FACS using specific markers, such as OCT4 and SSEA-1, 
      -3, and -4. Localization and in vivo transdifferentiation of injected cells in 
      the seminiferous tubules of recipients were traced for up to 40 days' 
      post-injection by GFP expression and immunocytochemical analyses. The integration 
      of the eGFP and the neo(R) genes in sperm gDNAs of recipient was confirmed via 
      PCR analysis. A subsequent testcross of the recipient roosters with non-Tg hens 
      resulted in the production of eGFP Tg progenies, demonstrating the successful 
      transdifferentiation of the adult BMC to the germ cells in the testis. Therefore, 
      we suggest that the use of adult BMCs is a new and promising approach to the 
      production of Tg poultry, and may prove helpful in the study of avian 
      developmental biology.
FAU - Heo, Young Tae
AU  - Heo YT
AD  - Department of Bioscience and Biotechnology, Bio-Organ Research Center, ARRC, 
      Konkuk University, Seoul, Korea.
FAU - Lee, Sung Ho
AU  - Lee SH
FAU - Yang, Ji Hoon
AU  - Yang JH
FAU - Kim, Teoan
AU  - Kim T
FAU - Lee, Hoon Taek
AU  - Lee HT
LA  - eng
PT  - Journal Article
DEP - 20110425
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
SB  - IM
MH  - Adult Stem Cells/physiology
MH  - Animals
MH  - *Animals, Genetically Modified
MH  - Bone Marrow Cells/*physiology
MH  - *Cell Transdifferentiation
MH  - Chickens/*genetics
MH  - Genetic Engineering
MH  - Male
MH  - Spermatozoa/*cytology
PMC - PMC3145912
EDAT- 2011/04/27 06:00
MHDA- 2011/09/29 06:00
CRDT- 2011/04/27 06:00
PHST- 2011/04/27 06:00 [entrez]
PHST- 2011/04/27 06:00 [pubmed]
PHST- 2011/09/29 06:00 [medline]
AID - S0023-6837(22)02746-5 [pii]
AID - 10.1038/labinvest.2011.53 [doi]
PST - ppublish
SO  - Lab Invest. 2011 Aug;91(8):1229-40. doi: 10.1038/labinvest.2011.53. Epub 2011 Apr 
      25.