PMID- 21520151
OWN - NLM
STAT- MEDLINE
DCOM- 20110929
LR  - 20180815
IS  - 1099-0844 (Electronic)
IS  - 0263-6484 (Linking)
VI  - 29
IP  - 4
DP  - 2011 Jun
TI  - VEGF-A-induced immature DCs not mature DCs differentiation into endothelial-like 
      cells through ERK1/2-dependent pathway.
PG  - 294-302
LID - 10.1002/cbf.1752 [doi]
AB  - Dendritic cells (DCs) are professional antigen-presenting cells that play an 
      important role in anti-tumour immunity. Endothelial-like differentiation of DCs 
      is an interesting phenomenon. The specific role of vascular endothelial growth 
      factor-A (VEGF-A) on the differentiation of immature DCs (iDCs) and mature DCs 
      (mDCs) is worth further research. Here, we show that VEGF-A can induce iDCs to 
      differentiate into endothelial-like cells (ELCs). But it has no obvious influence 
      on mDCs. In the process of endothelial-like differentiation of iDCs, a sustained 
      activation of extracellular signal-regulated kinase (ERK1/2) and cAMP response 
      element binding protein (CREB) was detected. VEGF-A induced the activation of 
      ERK1/2, and led to the nuclear translocation of phosphorylation ERK1/2. 
      Incubation of iDCs with the ERK1/2 upstream kinase MEK1/2 inhibitor PD98059, 
      blocked the phosphorylation of ERK1/2 and CREB as well as the endothelial-like 
      differentiation of iDCs. These data suggest that VEGF-A induces endothelial-like 
      differentiation of iDCs not mDCs through ERK1/2 signalling pathway.
CI  - Copyright (c) 2011 John Wiley & Sons, Ltd.
FAU - Lu, Jing
AU  - Lu J
AD  - Department of Pathophysiology, School of Basic Medical Sciences, Zhengzhou 
      University, Zhengzhou, China.
FAU - Zhao, Jun
AU  - Zhao J
FAU - Zhao, Jimin
AU  - Zhao J
FAU - Ma, Junfen
AU  - Ma J
FAU - Liu, Kangdong
AU  - Liu K
FAU - Yang, Hongyan
AU  - Yang H
FAU - Huang, Youtian
AU  - Huang Y
FAU - Qin, Zhenzhu
AU  - Qin Z
FAU - Bai, Ruihua
AU  - Bai R
FAU - Li, Pei
AU  - Li P
FAU - Yan, Wenhai
AU  - Yan W
FAU - Zhao, Mingyao
AU  - Zhao M
FAU - Dong, Ziming
AU  - Dong Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110425
PL  - England
TA  - Cell Biochem Funct
JT  - Cell biochemistry and function
JID - 8305874
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Flavonoids)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Active Transport, Cell Nucleus
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Cyclic AMP Response Element-Binding Protein/*metabolism
MH  - Dendritic Cells/cytology/drug effects/*physiology
MH  - Endothelial Cells/cytology/drug effects
MH  - Flavonoids/pharmacology
MH  - Flow Cytometry
MH  - Humans
MH  - Mitogen-Activated Protein Kinase 1/*metabolism
MH  - Mitogen-Activated Protein Kinase 3/*metabolism
MH  - Phosphorylation
MH  - Signal Transduction
MH  - Umbilical Veins/cytology
MH  - Vascular Endothelial Growth Factor A/*pharmacology
EDAT- 2011/04/27 06:00
MHDA- 2011/10/01 06:00
CRDT- 2011/04/27 06:00
PHST- 2010/11/30 00:00 [received]
PHST- 2011/02/14 00:00 [revised]
PHST- 2011/02/18 00:00 [accepted]
PHST- 2011/04/27 06:00 [entrez]
PHST- 2011/04/27 06:00 [pubmed]
PHST- 2011/10/01 06:00 [medline]
AID - 10.1002/cbf.1752 [doi]
PST - ppublish
SO  - Cell Biochem Funct. 2011 Jun;29(4):294-302. doi: 10.1002/cbf.1752. Epub 2011 Apr 
      25.