PMID- 21520275
OWN - NLM
STAT- MEDLINE
DCOM- 20111109
LR  - 20110722
IS  - 1523-4681 (Electronic)
IS  - 0884-0431 (Linking)
VI  - 26
IP  - 8
DP  - 2011 Aug
TI  - Central (ICV) leptin injection increases bone formation, bone mineral density, 
      muscle mass, serum IGF-1, and the expression of osteogenic genes in 
      leptin-deficient ob/ob mice.
PG  - 1710-20
LID - 10.1002/jbmr.406 [doi]
AB  - Both central and peripheral leptin administrations reduce body weight, food 
      intake, and adiposity in ob/ob mice. In this study we compared effects of 
      intracerebroventricular (ICV) and subcutaneous (SC) administration of leptin on 
      bone metabolism in the appendicular and axial skeleton and adipose tissue gene 
      expression and determined the effects of ICV leptin on bone marrow gene 
      expression in ob/ob mice. In experiment 1, leptin (1.5 or 0.38 microg/d) or control 
      was continuously injected ICV for 12 days. Gene expression analysis of femoral 
      bone marrow stromal cells showed that expression of genes associated with 
      osteogenesis was increased after ICV injection, whereas those associated with 
      osteoclastogenesis, adipogenesis, and adipocyte lipid storage were decreased. In 
      experiment 2, leptin was injected continuously ICV (0.0 or 1.5 microg/d) or SC (0.0 
      or 10 microg/d) for 12 days. In both experiments, regardless of mode of 
      administration, leptin decreased body weight, food intake, and body fat and 
      increased muscle mass, bone mineral density, bone mineral content, bone area, 
      marrow adipocyte number, and mineral apposition rate. Serum insulin was 
      decreased, whereas serum osteocalcin, insulin-like growth factor 1, 
      osteoprotegerin, pyridinoline, and receptor activator of nuclear factor kappaB ligand 
      concentrations were increased. In experiment 2, expression of genes in adipose 
      tissue associated with apoptosis, lipid mobilization, insulin sensitivity, and 
      thermogenesis was increased, whereas expression of genes associated with cell 
      differentiation and maturation was decreased regardless of mode of 
      administration. Thus ICV injection of leptin promotes expression of 
      pro-osteogenic factors in bone marrow, leading to enhanced bone formation in 
      ob/ob mice.
CI  - Copyright (c) 2011 American Society for Bone and Mineral Research.
FAU - Bartell, Shoshana M
AU  - Bartell SM
AD  - Department of Animal and Dairy Science, University of Georgia, Athens, GA 30602, 
      USA.
FAU - Rayalam, Srujana
AU  - Rayalam S
FAU - Ambati, Suresh
AU  - Ambati S
FAU - Gaddam, Dhanunjaya R
AU  - Gaddam DR
FAU - Hartzell, Diane L
AU  - Hartzell DL
FAU - Hamrick, Mark
AU  - Hamrick M
FAU - She, Jin-Xiong
AU  - She JX
FAU - Della-Fera, Mary Anne
AU  - Della-Fera MA
FAU - Baile, Clifton A
AU  - Baile CA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Bone Miner Res
JT  - Journal of bone and mineral research : the official journal of the American 
      Society for Bone and Mineral Research
JID - 8610640
RN  - 0 (Leptin)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
SB  - IM
CIN - J Bone Miner Res. 2011 Aug;26(8):1694-7. PMID: 21698664
MH  - Adipocytes/cytology/drug effects/metabolism
MH  - Animals
MH  - Body Weight/drug effects
MH  - Bone Density/*drug effects
MH  - Bone Marrow/drug effects/metabolism
MH  - Cell Count
MH  - Feeding Behavior/drug effects
MH  - Female
MH  - Femur/drug effects/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Injections, Intraventricular
MH  - Insulin-Like Growth Factor I/*metabolism
MH  - Leptin/administration & dosage/*deficiency/*pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Obese
MH  - Muscles/*anatomy & histology/drug effects
MH  - Organ Size/drug effects
MH  - Osteogenesis/*drug effects/*genetics
MH  - Tibia/anatomy & histology/drug effects/metabolism
EDAT- 2011/04/27 06:00
MHDA- 2011/11/10 06:00
CRDT- 2011/04/27 06:00
PHST- 2011/04/27 06:00 [entrez]
PHST- 2011/04/27 06:00 [pubmed]
PHST- 2011/11/10 06:00 [medline]
AID - 10.1002/jbmr.406 [doi]
PST - ppublish
SO  - J Bone Miner Res. 2011 Aug;26(8):1710-20. doi: 10.1002/jbmr.406.