PMID- 21521178
OWN - NLM
STAT- MEDLINE
DCOM- 20111028
LR  - 20220318
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 78
IP  - 1
DP  - 2011 Jul
TI  - Celiac disease and HLA typing using real-time PCR with melting curve analysis.
PG  - 31-7
LID - 10.1111/j.1399-0039.2011.01676.x [doi]
AB  - Celiac disease (CD) is an autoimmune disease characterized by chronic diarrhea, 
      inflammatory lesions of small bowel and nutritional malabsorption. CD is strongly 
      associated with the presence of HLA-DQB*02, DQB*03:02 and DQA*05. The absence of 
      any one of these three human leukocyte antigen (HLA) alleles rules out the 
      diagnosis of CD in suspected patients. Here, we describe a novel method to detect 
      the presence of these specific HLA alleles using real-time polymerase chain 
      reaction (PCR) with melting curve analysis. Compared with current HLA typing 
      assays, the real-time PCR method is faster, requires fewer handling steps and 
      provides 100% sensitivity and specificity for typing of HLA-DQB*02, DQB*03:02 and 
      DQA*05 alleles.
CI  - (c) 2011 John Wiley & Sons A/S.
FAU - Profaizer, T
AU  - Profaizer T
AD  - Histocompatibility & Immunogenetics Laboratory, University of Utah School of 
      Medicine, Salt Lake City, UT 84132-1904, USA. tracie.profaizer@hsc.utah.edu
FAU - Eckels, D
AU  - Eckels D
FAU - Delgado, J C
AU  - Delgado JC
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20110427
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ alpha-Chains)
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DQA1 antigen)
RN  - 0 (HLA-DQbeta antigen)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Celiac Disease/*genetics/immunology
MH  - Computer Systems
MH  - DNA/analysis
MH  - DNA Mutational Analysis/methods
MH  - HLA-DQ Antigens/analysis/genetics
MH  - HLA-DQ alpha-Chains
MH  - HLA-DQ beta-Chains
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Nucleic Acid Denaturation/genetics
MH  - Polymerase Chain Reaction/*methods
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Temperature
MH  - Transition Temperature
EDAT- 2011/04/28 06:00
MHDA- 2011/10/29 06:00
CRDT- 2011/04/28 06:00
PHST- 2011/04/28 06:00 [entrez]
PHST- 2011/04/28 06:00 [pubmed]
PHST- 2011/10/29 06:00 [medline]
AID - 10.1111/j.1399-0039.2011.01676.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2011 Jul;78(1):31-7. doi: 10.1111/j.1399-0039.2011.01676.x. Epub 
      2011 Apr 27.