PMID- 21521503
OWN - NLM
STAT- MEDLINE
DCOM- 20110909
LR  - 20211020
IS  - 1465-993X (Electronic)
IS  - 1465-9921 (Print)
IS  - 1465-9921 (Linking)
VI  - 12
IP  - 1
DP  - 2011 Apr 26
TI  - Ingraft chimerism in lung transplantation--a study in a porcine model of 
      obliterative bronchiolitis.
PG  - 56
LID - 10.1186/1465-9921-12-56 [doi]
AB  - BACKGROUND: Bronchial epithelium is a target of the alloimmune response in lung 
      transplantation, and intact epithelium may protect allografts from rejection and 
      obliterative bronchiolitis (OB). Herein we study the influence of chimerism on 
      bronchial epithelium and OB development in pigs. METHODS: A total of 54 
      immunosuppressed and unimmunosuppressed bronchial allografts were serially 
      obtained 2-90 days after transplantation. Histology (H&E) was assessed and the 
      fluorescence in situ hybridization (FISH) method for Y chromosomes using 
      pig-specific DNA-label was used to detect recipient derived cells in graft 
      epithelium and bronchial wall, and donor cell migration to recipient organs. 
      Ingraft chimerism was studied by using male recipients with female donors, 
      whereas donor cell migration to recipient organs was studied using female 
      recipients with male donors. RESULTS: Early appearance of recipient-derived cells 
      in the airway epithelium appeared predictive of epithelial destruction 
      (R=0.610-0.671 and p<0.05) and of obliteration of the bronchial lumen (R=0.698 
      and p<0.01). All allografts with preserved epithelium showed epithelial chimerism 
      throughout the follow-up. Antirejection medication did not prevent, but delayed 
      the appearance of Y chromosome positive cells in the epithelium (p<0.05), or 
      bronchial wall (p<0.05). CONCLUSIONS: In this study we demonstrate that early 
      appearance of Y chromosomes in the airway epithelium predicts features 
      characteristic of OB. Chimerism occurred in all allografts, including those 
      without features of OB. Therefore we suggest that ingraft chimerism may be a 
      mechanism involved in the repair of alloimmune-mediated tissue injury after 
      transplantation.
FAU - Paivaniemi, Outi E
AU  - Paivaniemi OE
AD  - Department of Cardiothoracic Surgery, Helsinki University Hospital, University of 
      Helsinki, P.O. Box 340, 00029 HUS, Helsinki, Finland. outi.paivaniemi@fimnet.fi
FAU - Musilova, Petra
AU  - Musilova P
FAU - Raivio, Peter M
AU  - Raivio PM
FAU - Maasilta, Paula K
AU  - Maasilta PK
FAU - Alho, Hanni S
AU  - Alho HS
FAU - Rubes, Jiri
AU  - Rubes J
FAU - Aittomaki, Kristiina
AU  - Aittomaki K
FAU - Salminen, Ulla-Stina
AU  - Salminen US
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110426
PL  - England
TA  - Respir Res
JT  - Respiratory research
JID - 101090633
RN  - 0 (Genetic Markers)
RN  - 0 (Immunosuppressive Agents)
SB  - IM
MH  - Animals
MH  - Bronchi/drug effects/immunology/pathology/*transplantation
MH  - Bronchiolitis Obliterans/genetics/*immunology/pathology/prevention & control
MH  - *Cell Movement
MH  - Disease Models, Animal
MH  - Female
MH  - Genetic Markers
MH  - Graft Rejection/genetics/*immunology/pathology/prevention & control
MH  - Immunosuppressive Agents/pharmacology
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Transplantation/*immunology
MH  - Male
MH  - Respiratory Mucosa/drug effects/immunology/pathology/*transplantation
MH  - Staining and Labeling
MH  - Sus scrofa
MH  - Time Factors
MH  - *Transplantation Chimera
MH  - Transplantation Tolerance
MH  - Transplantation, Homologous
MH  - Y Chromosome
PMC - PMC3111361
EDAT- 2011/04/28 06:00
MHDA- 2011/09/10 06:00
PMCR- 2011/04/26
CRDT- 2011/04/28 06:00
PHST- 2010/12/06 00:00 [received]
PHST- 2011/04/26 00:00 [accepted]
PHST- 2011/04/28 06:00 [entrez]
PHST- 2011/04/28 06:00 [pubmed]
PHST- 2011/09/10 06:00 [medline]
PHST- 2011/04/26 00:00 [pmc-release]
AID - 1465-9921-12-56 [pii]
AID - 10.1186/1465-9921-12-56 [doi]
PST - epublish
SO  - Respir Res. 2011 Apr 26;12(1):56. doi: 10.1186/1465-9921-12-56.