PMID- 21521635
OWN - NLM
STAT- MEDLINE
DCOM- 20110802
LR  - 20190625
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 408
IP  - 4
DP  - 2011 May 20
TI  - Cloning and heterologous expression of new xANO2 from Xenopus laevis.
PG  - 559-65
LID - 10.1016/j.bbrc.2011.04.060 [doi]
AB  - We have successfully isolated a novel anoctamin (xANO2), Ca(2+)-activated 
      chloride channel (ANO1, TMEM16A), from Xenopus laevis. The cDNA sequence was 
      determined to belong to the anoctamin family by comparison with the xTMEM16A 
      sequence in a previous report. Full length cDNA synthesis was performed by 
      repeating 5'- and 3'-rapid amplification of cDNA end (RACE). We successfully 
      completed the entire cDNA sequence and transiently named this sequence xANO2. The 
      xANO2 cDNA is 3884 base pair (bp) long and codes 980 amino acid (aa) proteins. 
      According to an aa homology search using the Basic Local Alignment Search Tool 
      (BLAST), xANO2 showed an overall identity of 92% to xTMEM16A (xANO1) 
      independently sub-cloned in our laboratory. A primary sequence of xANO2 revealed 
      typical characteristics of transmembrane proteins. In tissue distribution 
      analysis, the gene products of anoctamins were ubiquitously detected by real-time 
      PCR (RT-PCR). The expression profiles of each anoctamin were different among 
      brain, oocytes, and digestive organs with relatively weak expression. To clarify 
      the anoctamin activity, physiological studies were performed using the whole cell 
      patch-clamp technique with HEK293T cells, enhanced green fluorescent protein 
      (EGFP), and expression vectors carrying anoctamins. Characteristics typical of 
      voltage-dependent chloride currents were detected in cells expressing both xANO2 
      and xTMEM16A but not with EGFP alone. Sensitive reactions to the anion channel 
      blocker niflumic acid (NFA) were also revealed. Considering these results, xANO2 
      was regarded as a new TMEM16A belonging to the Xenopus anoctamin family.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Ryu, Rae Hyung
AU  - Ryu RH
AD  - Department of Biochemistry and Molecular Cell Biology, College of Veterinary 
      Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, Republic of 
      Korea.
FAU - Oh, Soo Jin
AU  - Oh SJ
FAU - Lee, Ra Mi
AU  - Lee RM
FAU - Jeong, Seong Won
AU  - Jeong SW
FAU - Jan, Lily Yeh
AU  - Jan LY
FAU - Lee, Chi Ho
AU  - Lee CH
FAU - Lee, C Justin
AU  - Lee CJ
FAU - Jeong, Sang Min
AU  - Jeong SM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110420
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Chloride Channels)
RN  - 0 (Xenopus Proteins)
RN  - 0 (xANO2 protein, Xenopus)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chloride Channels/chemistry/*genetics
MH  - Cloning, Molecular
MH  - Gene Expression
MH  - Molecular Sequence Data
MH  - Protein Conformation
MH  - Xenopus Proteins/chemistry/genetics
MH  - Xenopus laevis/*genetics
EDAT- 2011/04/28 06:00
MHDA- 2011/08/04 06:00
CRDT- 2011/04/28 06:00
PHST- 2011/04/09 00:00 [received]
PHST- 2011/04/14 00:00 [accepted]
PHST- 2011/04/28 06:00 [entrez]
PHST- 2011/04/28 06:00 [pubmed]
PHST- 2011/08/04 06:00 [medline]
AID - S0006-291X(11)00645-0 [pii]
AID - 10.1016/j.bbrc.2011.04.060 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2011 May 20;408(4):559-65. doi: 
      10.1016/j.bbrc.2011.04.060. Epub 2011 Apr 20.