PMID- 2152341
OWN - NLM
STAT- MEDLINE
DCOM- 19910118
LR  - 20190903
IS  - 0171-5216 (Print)
IS  - 0171-5216 (Linking)
VI  - 116
IP  - 6
DP  - 1990
TI  - Phenotypic patterns of preneoplastic and neoplastic hepatic lesions in woodchucks 
      infected with woodchuck hepatitis virus.
PG  - 581-90
AB  - Chronic infection of woodchucks with woodchuck hepatitis virus (WHV) was 
      associated with the development of hepatitis, foci of altered hepatocytes and 
      hepatocellular adenomas and carcinomas. The cytomorphological and cytochemical 
      analysis permitted the identification of three different types of focal lesions; 
      namely, glycogen-storage foci, mixed-cell foci and intermediate-cell foci, each 
      showing a characteristic pattern. The cells of the glycogen-storage foci had 
      clear to acidophilic cytoplasm, and were overloaded with glycogen. They showed a 
      marked elevation in the activity of glucose-6-phosphate dehydrogenase (G6PDH) and 
      malate dehydrogenase (MDH), increased activity of succinate dehydrogenase (SDH), 
      glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and glycerol-3-phosphate 
      dehydrogenase (G3PDH), reduction in the activity of glycogen phosphorylase (PHO), 
      glucose-6-phosphatase (G6Pase), adenosine triphosphatase (ATPase) and adenyl 
      cyclase (ADC), and unchanged activity of glycogen synthase (SYN) and 
      gamma-glutamyl transferase (GGT). The mixed-cell foci mainly consisted of 
      basophilic cells poor in glycogen, but were intermingled with cells containing 
      glycogen. These foci were characterized by a marked decrease in activity of PHO, 
      SYN, G6Pase, G6PDH, ATPase and ADC, and increased activity of GGT, SDH, MDH and 
      GAPDH. The intermediate-cell foci consisted of cells with both basophilic and 
      glycogenotic cytoplasmic compartments, and showed a similar enzyme histochemical 
      profile to the mixed-cell foci, with slight differences in the degree of 
      elevation or reduction of some enzymes. The phenotypic similarities and the close 
      spatial relationship between the foci of altered hepatocytes, and the 
      hepatocellular adenomas and carcinomas in WHV-infected woodchucks, suggest that 
      these lesions are preneoplastic. The focal morphological and metabolic 
      aberrations emerging during hepatocarcinogenesis in WHV-infected woodchuck, are 
      in principle similar to those identified in the course of chemical 
      hepatocarcinogenesis in various species. The focal metabolic aberrations 
      apparently represent a general biological response of the liver parenchyma to 
      oncogenic agents and are closely linked to neoplastic transformation of the 
      hepatocytes.
FAU - Toshkov, I
AU  - Toshkov I
AD  - Abteilung fur Cytopathologie, Deutsches Krebsforschungszentrum, Heidelberg, 
      Federal Republic of Germany.
FAU - Hacker, H J
AU  - Hacker HJ
FAU - Roggendorf, M
AU  - Roggendorf M
FAU - Bannasch, P
AU  - Bannasch P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Cancer Res Clin Oncol
JT  - Journal of cancer research and clinical oncology
JID - 7902060
SB  - IM
MH  - Animals
MH  - Female
MH  - *Hepadnaviridae
MH  - Hepatitis, Viral, Animal/*pathology
MH  - Liver/pathology
MH  - Liver Neoplasms/pathology/*veterinary
MH  - Male
MH  - *Marmota
MH  - Phenotype
MH  - Precancerous Conditions/*pathology
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1007/BF01637078 [doi]
PST - ppublish
SO  - J Cancer Res Clin Oncol. 1990;116(6):581-90. doi: 10.1007/BF01637078.