PMID- 21524945
OWN - NLM
STAT- MEDLINE
DCOM- 20120312
LR  - 20161125
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 879
IP  - 29
DP  - 2011 Nov 1
TI  - Effect of D-aspartate uptake on uncoupling protein-3 and alpha-tubulin expressions in 
      rat Harderian gland.
PG  - 3344-8
LID - 10.1016/j.jchromb.2011.04.001 [doi]
AB  - Although D-aspartate (D-Asp) has been recognized as having an important 
      physiological role within different organs, high concentrations could elicit 
      detrimental effects on those same organs. In this study, we evaluated the 
      oxidative stress response to D-Asp treatment in rat Harderian gland (HG) by 
      measuring total cellular hydroperoxide levels. Further, we examined the effect of 
      D-Asp uptake on the expression of the mitochondrial uncoupling protein-3 (UCP3), 
      beta-actin, and alpha-tubulin. In rat HG, elevated levels of D-Asp significantly 
      increased hydroperoxide production. This phenomenon was probably due to D-Asp 
      uptake as well as lipid and porphyrin increased levels. Higher UCP3 levels and 
      lower alpha-tubulin expression were also observed after D-Asp treatment. On the 
      contrary, beta-actin expression was unchanged. Given the possible role of UCP3 in 
      lipid handling, the higher expression of mitochondria UCP3 protein in 
      D-Asp-treated HG may reflect a major need to export excessive amounts of 
      hydroperoxides deriving from a greater fatty acid flux across these organelles 
      and higher mitochondrial porphyrin levels. Moreover, abundance of hydroperoxides 
      in D-Asp treated rat HG could determine the decrease of alpha-tubulin expression. 
      Thus, our findings indicate that a high concentration of D-Asp is critical in 
      initiating a cascade of events determined by oxidative stress.
CI  - Copyright (c) 2011. Published by Elsevier B.V.
FAU - Santillo, Alessandra
AU  - Santillo A
AD  - Dipartimento di Scienze della Vita, Seconda Universita di Napoli, via Vivaldi 43, 
      81100 Caserta, Italy.
FAU - Burrone, Lavinia
AU  - Burrone L
FAU - Senese, Rosalba
AU  - Senese R
FAU - Cioffi, Federica
AU  - Cioffi F
FAU - Lanni, Antonia
AU  - Lanni A
FAU - Chieffi Baccari, Gabriella
AU  - Chieffi Baccari G
LA  - eng
PT  - Journal Article
DEP - 20110412
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Actins)
RN  - 0 (Ion Channels)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Porphyrins)
RN  - 0 (Tubulin)
RN  - 0 (Ucp3 protein, rat)
RN  - 0 (Uncoupling Protein 3)
RN  - 4SR0Q8YD1X (D-Aspartic Acid)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Actins/analysis/metabolism
MH  - Animals
MH  - Blotting, Western
MH  - D-Aspartic Acid/pharmacokinetics/*pharmacology
MH  - Harderian Gland/*drug effects/*metabolism
MH  - Hydrogen Peroxide
MH  - Ion Channels/analysis/*metabolism
MH  - Lipid Metabolism
MH  - Male
MH  - Mitochondrial Proteins/analysis/*metabolism
MH  - Oxidative Stress/*drug effects
MH  - Porphyrins
MH  - Rats
MH  - Rats, Wistar
MH  - Tubulin/analysis/*metabolism
MH  - Uncoupling Protein 3
EDAT- 2011/04/29 06:00
MHDA- 2012/03/13 06:00
CRDT- 2011/04/29 06:00
PHST- 2010/11/29 00:00 [received]
PHST- 2011/03/23 00:00 [revised]
PHST- 2011/04/01 00:00 [accepted]
PHST- 2011/04/29 06:00 [entrez]
PHST- 2011/04/29 06:00 [pubmed]
PHST- 2012/03/13 06:00 [medline]
AID - S1570-0232(11)00238-8 [pii]
AID - 10.1016/j.jchromb.2011.04.001 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Nov 1;879(29):3344-8. doi: 
      10.1016/j.jchromb.2011.04.001. Epub 2011 Apr 12.