PMID- 21526121
OWN - NLM
STAT- MEDLINE
DCOM- 20110816
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 6
IP  - 4
DP  - 2011 Apr 22
TI  - Sex-linked pheromone receptor genes of the European corn borer, Ostrinia 
      nubilalis, are in tandem arrays.
PG  - e18843
LID - 10.1371/journal.pone.0018843 [doi]
LID - e18843
AB  - BACKGROUND: Tuning of the olfactory system of male moths to conspecific female 
      sex pheromones is crucial for correct species recognition; however, little is 
      known about the genetic changes that drive speciation in this system. Moths of 
      the genus Ostrinia are good models to elucidate this question, since significant 
      differences in pheromone blends are observed within and among species. Odorant 
      receptors (ORs) play a critical role in recognition of female sex pheromones; 
      eight types of OR genes expressed in male antennae were previously reported in 
      Ostrinia moths. METHODOLOGY/PRINCIPAL FINDINGS: We screened an O. nubilalis 
      bacterial artificial chromosome (BAC) library by PCR, and constructed three 
      contigs from isolated clones containing the reported OR genes. Fluorescence in 
      situ hybridization (FISH) analysis using these clones as probes demonstrated that 
      the largest contig, which contained eight OR genes, was located on the Z 
      chromosome; two others harboring two and one OR genes were found on two 
      autosomes. Sequence determination of BAC clones revealed the Z-linked OR genes 
      were closely related and tandemly arrayed; moreover, four of them shared 181-bp 
      direct repeats spanning exon 7 and intron 7. CONCLUSIONS/SIGNIFICANCE: This is 
      the first report of tandemly arrayed sex pheromone receptor genes in Lepidoptera. 
      The localization of an OR gene cluster on the Z chromosome agrees with previous 
      findings for a Z-linked locus responsible for O. nubilalis male behavioral 
      response to sex pheromone. The 181-bp direct repeats might enhance gene 
      duplications by unequal crossovers. An autosomal locus responsible for male 
      response to sex pheromone in Heliothis virescens and H. subflexa was recently 
      reported to contain at least four OR genes. Taken together, these findings 
      support the hypothesis that generation of additional copies of OR genes can 
      increase the potential for male moths to acquire altered specificity for 
      pheromone components, and accordingly, facilitate differentiation of sex 
      pheromones.
FAU - Yasukochi, Yuji
AU  - Yasukochi Y
AD  - Insect Genome Research Unit, National Institute of Agrobiological Sciences, 
      Tsukuba, Ibaraki, Japan. yaskoch@affrc.go.jp
FAU - Miura, Nami
AU  - Miura N
FAU - Nakano, Ryo
AU  - Nakano R
FAU - Sahara, Ken
AU  - Sahara K
FAU - Ishikawa, Yukio
AU  - Ishikawa Y
LA  - eng
SI  - GENBANK/AB597004
SI  - GENBANK/AB597005
SI  - GENBANK/AB597006
SI  - GENBANK/AB597007
SI  - GENBANK/AB597008
SI  - GENBANK/AB597304
SI  - GENBANK/AB597305
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110422
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Receptors, Odorant)
RN  - 0 (Receptors, Pheromone)
RN  - 0 (Sex Attractants)
SB  - IM
MH  - Animals
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Chromosomes, Insect/genetics
MH  - Europe
MH  - Exons/genetics
MH  - Female
MH  - Gene Duplication/genetics
MH  - Gene Order/genetics
MH  - Genes, Insect/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Introns/genetics
MH  - Lepidoptera/*genetics
MH  - Male
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Receptors, Odorant/genetics
MH  - Receptors, Pheromone/*genetics
MH  - Sequence Analysis, DNA
MH  - Sex Attractants/*genetics
MH  - Tandem Repeat Sequences/*genetics
MH  - Zea mays/*parasitology
PMC - PMC3081303
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2011/04/29 06:00
MHDA- 2011/08/17 06:00
PMCR- 2011/04/22
CRDT- 2011/04/29 06:00
PHST- 2010/10/26 00:00 [received]
PHST- 2011/03/21 00:00 [accepted]
PHST- 2011/04/29 06:00 [entrez]
PHST- 2011/04/29 06:00 [pubmed]
PHST- 2011/08/17 06:00 [medline]
PHST- 2011/04/22 00:00 [pmc-release]
AID - PONE-D-10-04291 [pii]
AID - 10.1371/journal.pone.0018843 [doi]
PST - epublish
SO  - PLoS One. 2011 Apr 22;6(4):e18843. doi: 10.1371/journal.pone.0018843.