PMID- 21529273
OWN - NLM
STAT- MEDLINE
DCOM- 20110913
LR  - 20151119
IS  - 1525-6049 (Electronic)
IS  - 0886-022X (Linking)
VI  - 33
IP  - 4
DP  - 2011
TI  - The effects of Syzygium aromaticum-derived oleanolic acid on glycogenic enzymes 
      in streptozotocin-induced diabetic rats.
PG  - 434-9
LID - 10.3109/0886022X.2011.568147 [doi]
AB  - Studies indicate that the antihyperglycemic effects of Syzygium 
      aromaticum-derived oleanolic acid (OA) are mediated in part through increased 
      hepatic glycogen synthesis. Accordingly, this study assessed the influence of OA 
      on the activity of glucokinase (GK) and hexokinase (HK) of skeletal muscle and 
      liver tissues in streptozotocin (STZ)-induced diabetic rats. After 5 weeks of OA 
      treatment, hepatic and gastrocnemius muscle glycogen concentrations and 
      activities of GK and HK were measured spectrophotometrically in reactions where 
      the oxidation of glucose-6-phosphate (G-6-PDH) formed was coupled to nicotinamide 
      adenine dinucleotide phosphate (NADP+) reduction catalyzed by G-6-PDH 
      dehydrogenase. Rats treated with deionized water or standard hypoglycemic drugs 
      acted as untreated and treated positive controls, respectively. STZ-induced 
      diabetic rats exhibited depleted glycogen levels and low activities of glycogenic 
      enzymes in muscle and hepatic tissues. OA administration restored these 
      biochemical alterations to near normalcy. The combination of OA and insulin did 
      not significantly alter the activities of HK and GK of STZ-induced diabetic rats, 
      suggesting that glycogen synthesis can also occur from precursors such as amino 
      acids or fructose and lactate. The attenuation of the activities of glycogenic 
      enzymes with concomitant increases of hepatic and muscle glycogen concentrations 
      of STZ-induced diabetic rats provides a therapeutic strategy for diabetes 
      treatment.
FAU - Ngubane, Phikelelani S
AU  - Ngubane PS
AD  - Department of Human PhysiologyFaculty of Health Science, School of Medical 
      Sciences, University of KwaZulu-Natal, Durban, South Africa.
FAU - Masola, Bubuya
AU  - Masola B
FAU - Musabayane, Cephas T
AU  - Musabayane CT
LA  - eng
PT  - Journal Article
PL  - England
TA  - Ren Fail
JT  - Renal failure
JID - 8701128
RN  - 0 (Plant Extracts)
RN  - 6SMK8R7TGJ (Oleanolic Acid)
RN  - 9005-79-2 (Glycogen)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.2 (Glucokinase)
SB  - IM
MH  - Animals
MH  - Carbohydrate Metabolism/*drug effects
MH  - Diabetes Mellitus, Experimental/*drug therapy
MH  - Glucokinase/metabolism
MH  - Glycogen/*metabolism
MH  - Hexokinase/metabolism
MH  - Liver/enzymology
MH  - Male
MH  - Muscle, Skeletal/enzymology
MH  - Oleanolic Acid/isolation & purification/pharmacology/*therapeutic use
MH  - *Phytotherapy
MH  - Plant Extracts/pharmacology/therapeutic use
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Syzygium/*chemistry
EDAT- 2011/05/03 06:00
MHDA- 2011/09/14 06:00
CRDT- 2011/05/03 06:00
PHST- 2011/05/03 06:00 [entrez]
PHST- 2011/05/03 06:00 [pubmed]
PHST- 2011/09/14 06:00 [medline]
AID - 10.3109/0886022X.2011.568147 [doi]
PST - ppublish
SO  - Ren Fail. 2011;33(4):434-9. doi: 10.3109/0886022X.2011.568147.