PMID- 2152940
OWN - NLM
STAT- MEDLINE
DCOM- 19900201
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 47
IP  - 1
DP  - 1990 Jan
TI  - Chemotactic peptide enhancement of phorbol ester-induced protein kinase C 
      activity in human neutrophils.
PG  - 49-59
AB  - Chemotactic factors and phorbol esters may act synergistically to evoke 
      neutrophil responses, but the mechanism of such interaction is not entirely 
      clear. We investigated the combined effects of the chemotactic peptide 
      n-formyl-methionyl-leucyl-phenylalanine (FMLP) and phorbol myristate acetate 
      (PMA) on protein kinase C (PKC) activity in human neutrophils. FMLP had little 
      effect on the sharp decrease in cytosolic PKC activity induced by PMA. However, 
      cells exposed to FMLP and PMA exhibited a synergistic increase in particulate PKC 
      activity (1 +/- 1 pmol 32P/10(7) PMNs/min in unstimulated cells, 53 +/- 12 pmol 
      32P with 20 ng/ml PMA, 6 +/- 3 pmol 32P with 10(-7) M FMLP, and 191 +/- 17 pmol 
      32P with FMLP and PMA). FMLP also markedly increased 
      calcium/phospholipid-independent protein kinase activity in particulate fractions 
      of control and PMA-treated cells. Enhancement of PKC activity required the 
      presence of cytochalasin B during cell stimulation. Cellular calcium was crucial 
      to the FMLP effect since enhancement was decreased in cells incubated with EGTA 
      or Quin2. These results suggest that chemotactic factors and phorbol esters may 
      mediate synergistic effects on neutrophil responses through enhancement of 
      particulate PKC activity. The enhancing effect is probably mediated through 
      chemoattractant-mediated increases in intracellular calcium.
FAU - Gay, J C
AU  - Gay JC
AD  - Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, 
      Tennessee.
FAU - Stitt, E S
AU  - Stitt ES
LA  - eng
GR  - AI 22383-01/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Diglycerides)
RN  - 0 (Isoquinolines)
RN  - 0 (Piperazines)
RN  - 11062-77-4 (Superoxides)
RN  - 3CHI920QS7 (Cytochalasin B)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 84477-87-2 (1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine)
RN  - 86390-77-4 (1-oleoyl-2-acetylglycerol)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
MH  - Calcium/pharmacology
MH  - Cytochalasin B/pharmacology
MH  - Diglycerides/pharmacology
MH  - Humans
MH  - Isoquinolines/pharmacology
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Neutrophils/drug effects/*enzymology
MH  - Phosphorylation
MH  - Piperazines/pharmacology
MH  - Protein Kinase C/*analysis/physiology
MH  - Superoxides/metabolism
MH  - Tetradecanoylphorbol Acetate/*pharmacology
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1002/jlb.47.1.49 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1990 Jan;47(1):49-59. doi: 10.1002/jlb.47.1.49.