PMID- 21530057
OWN - NLM
STAT- MEDLINE
DCOM- 20111024
LR  - 20110617
IS  - 1872-7654 (Electronic)
IS  - 0301-2115 (Linking)
VI  - 157
IP  - 1
DP  - 2011 Jul
TI  - Elevated C-reactive protein and monocyte chemoattractant protein-1 in patients 
      with polycystic ovary syndrome.
PG  - 53-6
LID - 10.1016/j.ejogrb.2011.03.015 [doi]
AB  - OBJECTIVES: C-reactive protein (CRP) and monocyte chemoattractant protein-1 
      (MCP-1) are inflammatory factors involved in the pathogenesis of atherosclerosis. 
      This study aimed to analyse serum concentrations of CRP and MCP-1 in patients 
      with polycystic ovary syndrome (PCOS), and to test the effect of these factors on 
      human THP-1 cells to determine the inflammatory state of these patients. STUDY 
      DESIGN: Eighty-five women with PCOS and 65 healthy women with regular menstrual 
      cycles, matched for age and body mass index (BMI), were included in the study. 
      Serum total cholesterol, triglycerides, high-density lipoprotein cholesterol 
      (HDL-C), low-density lipoprotein-cholesterol, fasting glucose and fasting insulin 
      were monitored. Serum CRP and MCP-1 were compared between patients with PCOS and 
      controls. Phorbol 12-myristate 13-acetate was used to induce the differentiation 
      of human THP-1 monocytes into THP-1 macrophages. THP-1 macrophages were incubated 
      with serum from patients with PCOS or controls for 24h, and MCP-1 mRNA expression 
      was determined using real-time reverse transcriptase polymerase chain reaction. 
      RESULTS: Serum CRP and MCP-1 levels were significantly higher in women with PCOS 
      compared with controls (CRP 1.9+/-2.0 mg/l vs. 0.9+/-0.8 mg/l; MCP-1 125.7+/-68.0 ng/ml 
      vs. 89.8+/-68.3 ng/ml, respectively). Serum CRP level was positively correlated 
      with BMI (r=0.494, p=0.000), waist:hip ratio (r=0.451, p=0.000), testosterone 
      (r=0.214, p=0.032), homeostasis model assessment of insulin resistance (HOMA-IR; 
      r=0.617, p=0.000) and MCP-1 (r=0.219, p=0.027), and negatively correlated with 
      HDL-C (r=-0.209, p=0.035). MCP-1 level was positively correlated with BMI 
      (r=0.381, p=0.000), waist:hip ratio (r=0.421, p=0.000), HOMA-IR (r=0.265, 
      p=0.007) and triglycerides (r=0.439, p=0.000). MCP-1 mRNA expression in THP-1 
      cells incubated with serum from patients with PCOS was significantly higher than 
      that in THP-1 cells incubated with serum from controls (p<0.05). CONCLUSION: 
      Patients with PCOS suffer low-grade chronic inflammation indicated by higher 
      levels of CRP and MCP-1, which could lead to increased risk of atherogenesis.
CI  - Copyright (c) 2011 Elsevier Ireland Ltd. All rights reserved.
FAU - Hu, Weihong
AU  - Hu W
AD  - Department of Gynaecology and Obstetrics, Peking University Third Hospital, 
      Beijing 100083, China.
FAU - Qiao, Jie
AU  - Qiao J
FAU - Yang, Yan
AU  - Yang Y
FAU - Wang, Lina
AU  - Wang L
FAU - Li, Rong
AU  - Li R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110429
PL  - Ireland
TA  - Eur J Obstet Gynecol Reprod Biol
JT  - European journal of obstetrics, gynecology, and reproductive biology
JID - 0375672
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 9007-41-4 (C-Reactive Protein)
SB  - IM
MH  - Adult
MH  - C-Reactive Protein/*analysis
MH  - Cell Line
MH  - Chemokine CCL2/*blood/genetics/metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - Hyperandrogenism/etiology
MH  - Hyperinsulinism/etiology
MH  - Hyperlipidemias/etiology
MH  - Insulin Resistance
MH  - Macrophage Activation
MH  - Macrophages/immunology/metabolism
MH  - Obesity, Abdominal/etiology
MH  - Polycystic Ovary Syndrome/*blood/*immunology/physiopathology
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Serum/immunology/metabolism
MH  - Severity of Illness Index
EDAT- 2011/05/03 06:00
MHDA- 2011/10/25 06:00
CRDT- 2011/05/03 06:00
PHST- 2010/02/23 00:00 [received]
PHST- 2010/07/30 00:00 [revised]
PHST- 2011/03/18 00:00 [accepted]
PHST- 2011/05/03 06:00 [entrez]
PHST- 2011/05/03 06:00 [pubmed]
PHST- 2011/10/25 06:00 [medline]
AID - S0301-2115(11)00173-4 [pii]
AID - 10.1016/j.ejogrb.2011.03.015 [doi]
PST - ppublish
SO  - Eur J Obstet Gynecol Reprod Biol. 2011 Jul;157(1):53-6. doi: 
      10.1016/j.ejogrb.2011.03.015. Epub 2011 Apr 29.