PMID- 21530973
OWN - NLM
STAT- MEDLINE
DCOM- 20110829
LR  - 20231213
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1218
IP  - 23
DP  - 2011 Jun 10
TI  - Rapid determination of glyphosate, glufosinate, bialaphos, and their major 
      metabolites in serum by liquid chromatography-tandem mass spectrometry using 
      hydrophilic interaction chromatography.
PG  - 3675-80
LID - 10.1016/j.chroma.2011.04.021 [doi]
AB  - We developed a simple and rapid method for the simultaneous determination of 
      phosphorus-containing amino acid herbicides (glyphosate, glufosinate, bialaphos) 
      and their major metabolites, aminomethylphosphonic acid (AMPA) and 
      3-methylphosphinicopropionic acid (MPPA), in human serum. Serum samples were 
      filtrated through an ultrafiltration membrane to remove proteins. The filtrate 
      was then washed with chloroform, and injected into a liquid chromatography-tandem 
      mass spectrometry (LC-MS/MS) system. Chromatographic separation was achieved on a 
      hydrophilic interaction chromatography (HILIC) column. Determination of the 
      target herbicides and metabolites was successfully carried out without 
      derivatization or solid phase extraction (SPE) cartridge clean-up. The recoveries 
      of these compounds, added to human serum at 0.2mug/mL, ranged from 94% to 108%, 
      and the relative standard deviations (RSDs) were within 5.9%. The limits of 
      detection (LODs) were 0.01mug/mL for MPPA, 0.02mug/mL for AMPA, 0.03mug/mL for both 
      glyphosate and glufosinate, and 0.07mug/mL for bialaphos, respectively.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Yoshioka, Naoki
AU  - Yoshioka N
AD  - Hyogo Prefectural Institute of Public Health and Consumer Sciences, 2-1-29, 
      Arata-cho, Hyogo-ku, Kobe 652-0032, Japan. Naoki Yoshioka@pref.hyogo.lg.jp
FAU - Asano, Migiwa
AU  - Asano M
FAU - Kuse, Azumi
AU  - Kuse A
FAU - Mitsuhashi, Takao
AU  - Mitsuhashi T
FAU - Nagasaki, Yasushi
AU  - Nagasaki Y
FAU - Ueno, Yasuhiro
AU  - Ueno Y
LA  - eng
PT  - Journal Article
DEP - 20110414
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (AE F061517)
RN  - 0 (Aminobutyrates)
RN  - 0 (Herbicides)
RN  - 0 (Isoxazoles)
RN  - 0 (Organophosphonates)
RN  - 0 (Organophosphorus Compounds)
RN  - 0 (Propionates)
RN  - 0 (Tetrazoles)
RN  - 35597-43-4 (bialaphos)
RN  - 51276-47-2 (phosphinothricin)
RN  - J090588E6X 
      (2-amino-3-(3-hydrox-5(2-methyl-2H-tetrazol-5-yl)isoxazol-4-yl)propionic acid)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Aminobutyrates/blood
MH  - Chromatography, Liquid/*methods
MH  - Female
MH  - Forensic Medicine
MH  - Glycine/analogs & derivatives/blood
MH  - Herbicides/*blood
MH  - Humans
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Isoxazoles
MH  - Male
MH  - Middle Aged
MH  - Organophosphonates/blood
MH  - Organophosphorus Compounds/blood
MH  - Propionates/blood
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Solid Phase Extraction
MH  - Tandem Mass Spectrometry/*methods
MH  - Tetrazoles
MH  - Glyphosate
EDAT- 2011/05/03 06:00
MHDA- 2011/08/30 06:00
CRDT- 2011/05/03 06:00
PHST- 2011/01/11 00:00 [received]
PHST- 2011/03/30 00:00 [revised]
PHST- 2011/04/07 00:00 [accepted]
PHST- 2011/05/03 06:00 [entrez]
PHST- 2011/05/03 06:00 [pubmed]
PHST- 2011/08/30 06:00 [medline]
AID - S0021-9673(11)00500-0 [pii]
AID - 10.1016/j.chroma.2011.04.021 [doi]
PST - ppublish
SO  - J Chromatogr A. 2011 Jun 10;1218(23):3675-80. doi: 10.1016/j.chroma.2011.04.021. 
      Epub 2011 Apr 14.