PMID- 21532169
OWN - NLM
STAT- MEDLINE
DCOM- 20110921
LR  - 20190720
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 34
IP  - 5
DP  - 2011
TI  - Rab5a regulates surface expression of FcepsilonRI and functional activation in mast 
      cells.
PG  - 760-3
AB  - Surface expression levels of high-affinity immunoglobulin E (IgE) receptors 
      (FcepsilonRI) on mast cells are regulated by constitutive internalization from the 
      plasma membrane, which is thought to be an important determinant of 
      FcepsilonRI-mediated signaling potential. However, molecular mechanism of FcepsilonRI 
      trafficking has remained poorly understood. Rab proteins are small guanosine 
      5'-triphosphatases (GTPases) involved in the regulation of membrane traffic. In 
      particular, Rab5 has been shown to regulate transport in the early endocytic 
      pathway, whereas it is not known whether the FcepsilonRI surface expression levels are 
      regulated by Rab5. In this study, we investigated the role of individual Rab5 
      isoforms in mast cells by small interfering RNA knockdown method. Our results 
      demonstrate that Rab5a knockdown enhanced FcepsilonRI-dependent mast cell activation 
      and upregulated FcepsilonRI surface expression in its steady state. In contrast, Rab5c 
      knockdown caused suppression of the activation. These findings revealed 
      modulatory and individual roles of Rab5 isoforms in mast cell functions.
FAU - Kageyama-Yahara, Natsuko
AU  - Kageyama-Yahara N
AD  - Division of Gastrointestinal Pathophysiology, Department of Bioscience, Institute 
      of Natural Medicine, University of Toyama, Toyama, Japan. 
      natsukokageyama-tky@umin.ac.jp
FAU - Suehiro, Yoko
AU  - Suehiro Y
FAU - Yamamoto, Takeshi
AU  - Yamamoto T
FAU - Kadowaki, Makoto
AU  - Kadowaki M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (DNA Primers)
RN  - 0 (Receptors, IgE)
RN  - EC 3.6.5.2 (rab5 GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - DNA Primers
MH  - Gene Knockdown Techniques
MH  - Mast Cells/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Receptors, IgE/*metabolism
MH  - rab5 GTP-Binding Proteins/genetics/*physiology
EDAT- 2011/05/03 06:00
MHDA- 2011/09/22 06:00
CRDT- 2011/05/03 06:00
PHST- 2011/05/03 06:00 [entrez]
PHST- 2011/05/03 06:00 [pubmed]
PHST- 2011/09/22 06:00 [medline]
AID - JST.JSTAGE/bpb/34.760 [pii]
AID - 10.1248/bpb.34.760 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2011;34(5):760-3. doi: 10.1248/bpb.34.760.