PMID- 21532279
OWN - NLM
STAT- MEDLINE
DCOM- 20111213
LR  - 20190724
IS  - 1347-5231 (Electronic)
IS  - 0031-6903 (Linking)
VI  - 131
IP  - 5
DP  - 2011
TI  - [Simple and rapid screening for methamphetamine and 
      3,4-methylenedioxymethamphetamine (MDMA) and their metabolites in urine using 
      direct analysis in real time (DART)-TOFMS].
PG  - 827-33
AB  - An ionization technique, direct analysis in real time (DART) has recently been 
      developed for the ambient ionization of a variety samples. The DART coupled with 
      time-of-flight mass spectrometry (TOFMS) would be useful as a simple and rapid 
      screening for the targeted compounds in various samples, because it provides the 
      molecular information of these compounds without time-consuming extraction. In 
      this study, we investigated rapid screening methods of illicit drugs and their 
      metabolites, such as methamphetamine (MA), 3,4-methylenedioxymethamphetamine 
      (MDMA), amphetamine (AP) and 3,4-methylenedioxyamphetamine (MDA) in human urine 
      using DART-TOFMS. As serious matrix effects caused by urea in urine samples and 
      ionizations of the targeted compounds were greatly suppressed in the DART-TOFMS 
      analyses, simple pretreatment methods to remove the urea from the samples were 
      investigated. When a pipette tip-type solid-phase extraction with a 
      dichloromethane and isopropanol mixed solution as an eluent was used for the 
      pretreatment, the limits of detection (LODs) of 4 compounds added to control 
      urine samples were 0.25 microg/ml. On the other hand, the LODs of these compounds 
      were 0.5 microg/ml by a liquid-liquid extraction using a dichloromethane and hexane 
      mixed solution. In both extractions, the recoveries of 4 compounds from urine 
      samples were over 70% and these extraction methods showed good linearity in the 
      range of 0.5-5 microg/ml by GC-MS analyses. In conclusion, our proposed method using 
      DART-TOFMS could simultaneously detect MA, MDMA and their metabolites in urine at 
      0.5 microg/ml without time-consuming pretreatment steps. Therefore it would be useful 
      for screening drugs in urine with the molecular information.
FAU - Kawamura, Maiko
AU  - Kawamura M
AD  - National Institute of Health Sciences, Tokyo, Japan.
FAU - Kikura-Hanajiri, Ruri
AU  - Kikura-Hanajiri R
FAU - Goda, Yukihiro
AU  - Goda Y
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Yakugaku Zasshi
JT  - Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
JID - 0413613
RN  - 44RAL3456C (Methamphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Humans
MH  - Mass Spectrometry/*methods
MH  - Methamphetamine/*urine
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*urine
MH  - Substance Abuse Detection/*methods
EDAT- 2011/05/03 06:00
MHDA- 2011/12/14 06:00
CRDT- 2011/05/03 06:00
PHST- 2011/05/03 06:00 [entrez]
PHST- 2011/05/03 06:00 [pubmed]
PHST- 2011/12/14 06:00 [medline]
AID - JST.JSTAGE/yakushi/131.827 [pii]
AID - 10.1248/yakushi.131.827 [doi]
PST - ppublish
SO  - Yakugaku Zasshi. 2011;131(5):827-33. doi: 10.1248/yakushi.131.827.