PMID- 21533221
OWN - NLM
STAT- MEDLINE
DCOM- 20110812
LR  - 20211020
IS  - 1553-7404 (Electronic)
IS  - 1553-7390 (Print)
IS  - 1553-7390 (Linking)
VI  - 7
IP  - 4
DP  - 2011 Apr
TI  - Genome analysis reveals interplay between 5'UTR introns and nuclear mRNA export 
      for secretory and mitochondrial genes.
PG  - e1001366
LID - 10.1371/journal.pgen.1001366 [doi]
LID - e1001366
AB  - In higher eukaryotes, messenger RNAs (mRNAs) are exported from the nucleus to the 
      cytoplasm via factors deposited near the 5' end of the transcript during 
      splicing. The signal sequence coding region (SSCR) can support an alternative 
      mRNA export (ALREX) pathway that does not require splicing. However, most 
      SSCR-containing genes also have introns, so the interplay between these export 
      mechanisms remains unclear. Here we support a model in which the furthest 
      upstream element in a given transcript, be it an intron or an ALREX-promoting 
      SSCR, dictates the mRNA export pathway used. We also experimentally demonstrate 
      that nuclear-encoded mitochondrial genes can use the ALREX pathway. Thus, ALREX 
      can also be supported by nucleotide signals within mitochondrial-targeting 
      sequence coding regions (MSCRs). Finally, we identified and experimentally 
      verified novel motifs associated with the ALREX pathway that are shared by both 
      SSCRs and MSCRs. Our results show strong correlation between 5' untranslated 
      region (5'UTR) intron presence/absence and sequence features at the beginning of 
      the coding region. They also suggest that genes encoding secretory and 
      mitochondrial proteins share a common regulatory mechanism at the level of mRNA 
      export.
FAU - Cenik, Can
AU  - Cenik C
AD  - Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical 
      School, Boston, Massachusetts, United States of America.
FAU - Chua, Hon Nian
AU  - Chua HN
FAU - Zhang, Hui
AU  - Zhang H
FAU - Tarnawsky, Stefan P
AU  - Tarnawsky SP
FAU - Akef, Abdalla
AU  - Akef A
FAU - Derti, Adnan
AU  - Derti A
FAU - Tasan, Murat
AU  - Tasan M
FAU - Moore, Melissa J
AU  - Moore MJ
FAU - Palazzo, Alexander F
AU  - Palazzo AF
FAU - Roth, Frederick P
AU  - Roth FP
LA  - eng
GR  - R01 HG001715/HG/NHGRI NIH HHS/United States
GR  - R21 MH087394/MH/NIMH NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - NS035611/NS/NINDS NIH HHS/United States
GR  - U01 HG001715/HG/NHGRI NIH HHS/United States
GR  - HG003224/HG/NHGRI NIH HHS/United States
GR  - U01 HL081341/HL/NHLBI NIH HHS/United States
GR  - U41 HG001715/HG/NHGRI NIH HHS/United States
GR  - HG004233/HG/NHGRI NIH HHS/United States
GR  - MH087394/MH/NIMH NIH HHS/United States
GR  - P01 NS035611/NS/NINDS NIH HHS/United States
GR  - HL081341/HL/NHLBI NIH HHS/United States
GR  - P50 HG004233/HG/NHGRI NIH HHS/United States
GR  - R01 HG003224/HG/NHGRI NIH HHS/United States
GR  - HG001715/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110414
PL  - United States
TA  - PLoS Genet
JT  - PLoS genetics
JID - 101239074
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Protein Sorting Signals)
RN  - 0 (RNA, Messenger)
RN  - JAC85A2161 (Adenine)
SB  - IM
MH  - 5' Untranslated Regions/*genetics
MH  - Active Transport, Cell Nucleus
MH  - Adenine/metabolism
MH  - *Alternative Splicing
MH  - Cell Nucleus/*metabolism
MH  - Cytoplasm
MH  - Endoplasmic Reticulum/genetics
MH  - Gene Expression Regulation
MH  - Genes, Mitochondrial
MH  - Humans
MH  - Introns
MH  - Models, Genetic
MH  - Open Reading Frames
MH  - Protein Sorting Signals
MH  - RNA Splicing
MH  - *RNA Transport
MH  - RNA, Messenger/*metabolism
PMC - PMC3077370
COIS- The authors have declared that no competing interests exist.
EDAT- 2011/05/03 06:00
MHDA- 2011/08/13 06:00
PMCR- 2011/04/01
CRDT- 2011/05/03 06:00
PHST- 2010/08/09 00:00 [received]
PHST- 2011/03/07 00:00 [accepted]
PHST- 2011/05/03 06:00 [entrez]
PHST- 2011/05/03 06:00 [pubmed]
PHST- 2011/08/13 06:00 [medline]
PHST- 2011/04/01 00:00 [pmc-release]
AID - 10-PLGE-RA-3857R2 [pii]
AID - 10.1371/journal.pgen.1001366 [doi]
PST - ppublish
SO  - PLoS Genet. 2011 Apr;7(4):e1001366. doi: 10.1371/journal.pgen.1001366. Epub 2011 
      Apr 14.