PMID- 21533384
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130704
LR  - 20190606
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 10
IP  - 2
DP  - 1997 Feb
TI  - Peritoneal dissemination is inhibited by treatment with antibodies against CD44H, 
      beta(1) integrin, and carcinostatic agents in NUGC-4 human gastric cancer cells.
PG  - 355-62
AB  - Peritoneal dissemination frequently occurs after surgery in patients with gastric 
      cancer. The presence of peritoneal metastasis after surgery affects the 
      prognosis, therefore, a way must be found to effectively prevent the development 
      of peritoneal dissemination. Very little is known about the biochemical processes 
      involved in the initial attachment of gastric cancer cells to peritoneal 
      mesothelial cells. We conducted in vitro and in vivo studies to assess the role 
      of adhesion molecules and TGF-beta 1 in this process, using 4 gastric cancer cell 
      lines. NUGC-4 cells, which disseminate early after inoculation into the abdominal 
      cavity of nude mice, predominantly expressed CD44H and beta(1) integrin. We found 
      that NUGC-4 cells adhered to monolayers of mesothelial cells more firmly than 
      other cell lines. Adhesion of NUGC-4 cells to mesothelial cells was partially 
      inhibited by antibodies against CD44H or the beta(1) subunit of integrin, and was 
      completely blocked by a combination of these 2 antibodies. Treatment with ligands 
      for CD44H and beta(1) integrin also inhibited this adhesion. In the NUGC-4 cell 
      culture medium, larger amounts of transforming growth factor beta 1(TGF-beta 1) 
      was detected in proportion to the increase in cancer cells than in the other cell 
      lines. TGF-beta 1 increased the expression of CD44H in NUGC-4 cells and in 
      mesothelial cells, and augmented the adhesion and implantation of NUGC-4 cells to 
      mesothelial cells accompanied by accumulation of extracellular matrix (ECM) 
      components. Carcinostatic agents decreased the expression of CD44H but increased 
      the expression of E-cadherin in NUGC-4 cells. Treatment with carcinostatic agents 
      and antibodies against CD44H and beta(1) integrin inhibited the dissemination of 
      NUGC-4 cells in the peritoneal cavity of nude mice, and prolonged their survival 
      time. These findings suggest that CD44H and integrins mediate in the initial 
      attachment of gastric cancer cells to mesothelial cells, and TGF-beta 1 
      participates in the promotion of the disease. It is possible that a treatment 
      strategy that interferes with CD44H or integrins function and increases the 
      functions of E-cadherin immediately after surgery may result in the decreased 
      intra-abdominal spread of gastric cancer.
FAU - Nakashio, T
AU  - Nakashio T
AD  - AICHI CANC CTR,RES INST,LAB EXPT PATHOL,NAGOYA,AICHI 464,JAPAN.
FAU - Narita, T
AU  - Narita T
FAU - Akiyama, S
AU  - Akiyama S
FAU - Kasai, Y
AU  - Kasai Y
FAU - Kondo, K
AU  - Kondo K
FAU - Ito, K
AU  - Ito K
FAU - Takagi, H
AU  - Takagi H
FAU - Kannagi, R
AU  - Kannagi R
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 2011/05/03 06:00
PHST- 2011/05/03 06:00 [entrez]
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
AID - 10.3892/ijo.10.2.355 [doi]
PST - ppublish
SO  - Int J Oncol. 1997 Feb;10(2):355-62. doi: 10.3892/ijo.10.2.355.