PMID- 21535022
OWN - NLM
STAT- MEDLINE
DCOM- 20111228
LR  - 20220408
IS  - 1365-2591 (Electronic)
IS  - 0143-2885 (Linking)
VI  - 44
IP  - 9
DP  - 2011 Sep
TI  - Potential systematic error in laboratory experiments on microbial leakage through 
      filled root canals: an experimental study.
PG  - 827-35
LID - 10.1111/j.1365-2591.2011.01888.x [doi]
AB  - AIM: To assess the routes of bacterial leakage in a commonly used two-chamber 
      model designed to evaluate root fillings. METHODOLOGY: Fifty-one intact human 
      mandibular premolars with fully developed roots were used. They were left 
      completely intact (n=23), or were accessed, instrumented and either left open 
      (n=5) or root filled with gutta-percha and AH Plus (n=23). All teeth were sealed 
      between two chambers using sticky wax. The apical root aspects were left 
      uncovered. The upper chamber was seeded with Enterococcus faecalis. An 
      enterococci-selective broth was used in the lower chamber. Leakage was assessed 
      for 120 days and compared using survival statistics (alpha<0.05). Subsequently, roots 
      were trans-sected, stained using a 'live' DNA stain (Syto59) and inspected using 
      confocal laser scanning microscopy. An E. faecalis-specific RNA probe was used 
      for fluorescence in situ hybridization (FISH). RESULTS: Leakage started to occur 
      from day 56, with further occurrence essentially identical between root filled 
      teeth and intact counterparts (P=0.71). All the trans-sections showed 
      fluorescence related to Syto59 between the cementum layer and the sticky wax. 
      Fluorescence was also observed between the root filling and the tubular dentine, 
      whilst it was absent at the interface between root filling and sclerotic dentine. 
      Secondary dentinal tubules, i.e. lateral branches connecting the main 
      counterparts, contained fluorescent material. FISH revealed that Syto59 
      exclusively stained E. faecalis. CONCLUSIONS: The current experimental method 
      proved to be unsuitable to compare root fillings. Histology revealed interesting 
      observations regarding the relationship of dentine structure and bacterial 
      leakage, which warrant further investigation.
CI  - (c) 2011 International Endodontic Journal.
FAU - Rechenberg, D-K
AU  - Rechenberg DK
AD  - Department of Preventive Dentistry, Periodontology and Cariology, University of 
      Zurich Center of Dental Medicine, Zurich, Switzerland. dan.rechenberg@zzm.uzh.ch
FAU - Thurnheer, T
AU  - Thurnheer T
FAU - Zehnder, M
AU  - Zehnder M
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
DEP - 20110502
PL  - England
TA  - Int Endod J
JT  - International endodontic journal
JID - 8004996
RN  - 0 (Root Canal Filling Materials)
MH  - Bicuspid
MH  - Dental Leakage/*diagnosis/microbiology
MH  - Dental Marginal Adaptation
MH  - Dental Pulp Cavity/*microbiology
MH  - Dental Research/*methods/standards
MH  - Dentin/*microbiology
MH  - Enterococcus faecalis
MH  - Humans
MH  - Mandible
MH  - Microscopy, Confocal
MH  - Models, Anatomic
MH  - Reproducibility of Results
MH  - *Root Canal Filling Materials
MH  - Tooth, Nonvital
EDAT- 2011/05/04 06:00
MHDA- 2011/12/29 06:00
CRDT- 2011/05/04 06:00
PHST- 2011/05/04 06:00 [entrez]
PHST- 2011/05/04 06:00 [pubmed]
PHST- 2011/12/29 06:00 [medline]
AID - 10.1111/j.1365-2591.2011.01888.x [doi]
PST - ppublish
SO  - Int Endod J. 2011 Sep;44(9):827-35. doi: 10.1111/j.1365-2591.2011.01888.x. Epub 
      2011 May 2.