PMID- 21536241
OWN - NLM
STAT- MEDLINE
DCOM- 20110706
LR  - 20220331
IS  - 2210-7762 (Print)
VI  - 204
IP  - 4
DP  - 2011 Apr
TI  - Evaluating chromosomal mosaicism by array comparative genomic hybridization in 
      hematological malignancies: the proposal of a formula.
PG  - 216-8
LID - 10.1016/j.cancergen.2011.02.002 [doi]
AB  - Array-based comparative genomic hybridization (aCGH) has proven indispensable to 
      the study of unbalanced constitutional and acquired chromosomal anomalies, but 
      its sensitivity for detecting mosaicism is still not well established. On the 
      basis of the ADM2 algorithm used for microarray image analysis with one of the 
      most widely used oligomer-based aCGH platforms [the whole genome 244K system by 
      Agilent Technologies (Santa Clara, CA)] we suggest a formula to infer the 
      percentage of cells bearing a chromosome imbalance in cases with constitutional 
      or acquired mosaicism. Three examples of acquired mosaicism in which this formula 
      was applied are reported together with parallel fluorescence in situ 
      hybridization (FISH) to interphase nuclei with informative probes. Although some 
      approximation affects both the results inferred from aCGH and FISH data, the 
      proposed formula was successful in the three patients studied.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Valli, Roberto
AU  - Valli R
AD  - Biologia e Genetica, Dipartimento di Scienze Biomediche Sperimentali e Cliniche, 
      Universita dell'Insubria, Varese, Italy.
FAU - Maserati, Emanuela
AU  - Maserati E
FAU - Marletta, Cristina
AU  - Marletta C
FAU - Pressato, Barbara
AU  - Pressato B
FAU - Lo Curto, Francesco
AU  - Lo Curto F
FAU - Pasquali, Francesco
AU  - Pasquali F
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet
JT  - Cancer genetics
JID - 101539150
SB  - IM
MH  - Algorithms
MH  - Comparative Genomic Hybridization/*methods
MH  - Hematologic Neoplasms/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Microarray Analysis
MH  - *Mosaicism
EDAT- 2011/05/04 06:00
MHDA- 2011/07/08 06:00
CRDT- 2011/05/04 06:00
PHST- 2010/12/06 00:00 [received]
PHST- 2011/01/26 00:00 [revised]
PHST- 2011/02/07 00:00 [accepted]
PHST- 2011/05/04 06:00 [entrez]
PHST- 2011/05/04 06:00 [pubmed]
PHST- 2011/07/08 06:00 [medline]
AID - S2210-7762(11)00021-4 [pii]
AID - 10.1016/j.cancergen.2011.02.002 [doi]
PST - ppublish
SO  - Cancer Genet. 2011 Apr;204(4):216-8. doi: 10.1016/j.cancergen.2011.02.002.