PMID- 21536256
OWN - NLM
STAT- MEDLINE
DCOM- 20111011
LR  - 20141120
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1394
DP  - 2011 Jun 7
TI  - Possible contribution of pannexin channel to ATP-induced currents in vitro in 
      vasopressin neurons isolated from the rat supraoptic nucleus.
PG  - 71-8
LID - 10.1016/j.brainres.2011.04.017 [doi]
AB  - Release of arginine vasopressin (AVP) from magnocellular neurosecretory cells 
      (MNCs) of the supraoptic nucleus (SON) is controlled by the electrical activity 
      of these neurons. ATP plays a crucial role in the regulation of SON MNCs by 
      activating the purinergic P2X and P2Y receptors. Recent reports of interaction 
      between P2X receptors and pannexin channels have provided new insights into the 
      physiology of the central nervous system; however, the function of pannexin 
      channels has not been assessed in AVP neurons. In the present study, we examined 
      the possible contribution of the pannexin channel in ATP-induced responses in SON 
      AVP neurons. We used the whole-cell patch-clamp technique in isolated rat SON 
      MNCs that express an AVP-enhanced green fluorescent protein transgene. The 
      ATP-induced current was inhibited in a concentration-dependent manner by pannexin 
      channel blockers carbenoxolone and mefloquine, whereas the connexin channel 
      blockers flufenamic acid and lanthanum had no effect. Multi-cell reverse 
      transcriptase-polymerase chain reaction experiments confirmed the existence of 
      pannexin-1 mRNA in AVP neurons. The involvement of the ATP-activated transient 
      receptor potential vanilloid and acid-sensing ion channels was excluded. These 
      results suggest that pannexin channels in SON AVP neurons are involved in the 
      regulatory mechanisms of neuronal activity.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Ohbuchi, Toyoaki
AU  - Ohbuchi T
AD  - Department of Physiology, School of Medicine, University of Occupational and 
      Environmental Health, Kitakyushu, Japan.
FAU - Yokoyama, Toru
AU  - Yokoyama T
FAU - Saito, Takeshi
AU  - Saito T
FAU - Ohkubo, Jun-Ichi
AU  - Ohkubo J
FAU - Suzuki, Hitoshi
AU  - Suzuki H
FAU - Ishikura, Toru
AU  - Ishikura T
FAU - Katoh, Akiko
AU  - Katoh A
FAU - Fujihara, Hiroaki
AU  - Fujihara H
FAU - Hashimoto, Hirofumi
AU  - Hashimoto H
FAU - Suzuki, Hideaki
AU  - Suzuki H
FAU - Ueta, Yoichi
AU  - Ueta Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110415
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Connexins)
RN  - 0 (Ion Channels)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (pannexin 1, rat)
RN  - 11000-17-2 (Vasopressins)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Connexins/metabolism
MH  - In Vitro Techniques
MH  - Ion Channels/*physiology
MH  - Male
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurons/*physiology
MH  - Patch-Clamp Techniques
MH  - Rats
MH  - Rats, Transgenic
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Supraoptic Nucleus/*physiology
MH  - Vasopressins/metabolism
EDAT- 2011/05/04 06:00
MHDA- 2011/10/12 06:00
CRDT- 2011/05/04 06:00
PHST- 2011/02/04 00:00 [received]
PHST- 2011/03/29 00:00 [revised]
PHST- 2011/04/10 00:00 [accepted]
PHST- 2011/05/04 06:00 [entrez]
PHST- 2011/05/04 06:00 [pubmed]
PHST- 2011/10/12 06:00 [medline]
AID - S0006-8993(11)00733-5 [pii]
AID - 10.1016/j.brainres.2011.04.017 [doi]
PST - ppublish
SO  - Brain Res. 2011 Jun 7;1394:71-8. doi: 10.1016/j.brainres.2011.04.017. Epub 2011 
      Apr 15.