PMID- 21537065
OWN - NLM
STAT- MEDLINE
DCOM- 20110810
LR  - 20211216
IS  - 1972-2680 (Electronic)
IS  - 1972-2680 (Linking)
VI  - 5
IP  - 4
DP  - 2011 Apr 26
TI  - Comparison of virulence markers and antibiotic resistance in enterotoxigenic 
      Escherichia coli isolated ten years apart in Tehran.
PG  - 248-54
AB  - INTRODUCTION: Enterotoxigenic Escherichia coli (ETEC) causes diarrhoea by 
      producing heat-labile (LT) or heat-stable (ST) enterotoxins after colonizing the 
      small intestine by means of colonization factors (CFs). Although detection of the 
      toxins is sufficient for verification of ETEC isolates, toxin-positive strains 
      may be further analyzed for the presence of CFs. Antibiotics may shorten the 
      duration of diarrhoea caused by ETEC, but the rapid emergence of resistant 
      strains limits their usefulness. METHODOLOGY: ETEC isolates collected 10 years 
      apart were compared for the prevalence of toxin types, CFs and antibiotic 
      resistance. DNA/DNA hybridization with digoxigenin (DIG)-labeled probes was used 
      for the detection of toxin types, and CF-typing was performed by DNA 
      hybridization using DIG-labeled probes for cfaD and CS6 with slide agglutination. 
      Disk diffusion was used to determine antibiotic resistance. The presence of class 
      1 integrons was detected by PCR. RESULTS: ST-positive isolates were the most 
      prevalent among the isolates from 1988, but a significant shift towards LT-gene 
      carriage was observed in the 1998 group. CFA/I and CFA/IV were the most common CF 
      types within both groups. The most prevalent resistance patterns among these 
      isolates were ACSTSXT followed by ASTSXT and ASSXT. CONCLUSION: Our study of the 
      two groups of isolates showed that the rate of LT and ST gene carriage, as well 
      as antibiotic resistance markers, has changed in the ten years separating the two 
      bacterial populations. These variations show the importance of monitoring 
      pathogenic bacteria to obtain a near realistic picture of the circulating 
      bacterial pathogens.
FAU - Shahrokhi, Nader
AU  - Shahrokhi N
AD  - Molecular Biology Unit, Pasteur Institute of Iran, Tehran 13164, Iran. 
      shahrokhi@pasteur.ac.ir
FAU - Bouzari, Saeid
AU  - Bouzari S
FAU - Jafari, Anis
AU  - Jafari A
LA  - eng
PT  - Journal Article
DEP - 20110426
PL  - Italy
TA  - J Infect Dev Ctries
JT  - Journal of infection in developing countries
JID - 101305410
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Toxins)
RN  - 0 (CS6 antigen, E coli)
RN  - 0 (CfaD protein, E coli)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Virulence Factors)
RN  - 0 (heat stable toxin (E coli))
RN  - D9K3SN2LNY (heat-labile enterotoxin, E coli)
SB  - IM
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Antigens, Bacterial/genetics
MH  - Bacterial Toxins/genetics
MH  - Child, Preschool
MH  - DNA, Bacterial/genetics
MH  - DNA-Binding Proteins/genetics
MH  - *Drug Resistance, Bacterial
MH  - Enterotoxigenic Escherichia coli/*drug effects/genetics/isolation & 
      purification/*pathogenicity
MH  - Enterotoxins/genetics
MH  - Escherichia coli Infections/*microbiology
MH  - Escherichia coli Proteins/genetics
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Iran
MH  - Microbial Sensitivity Tests
MH  - Nucleic Acid Hybridization
MH  - Polymerase Chain Reaction
MH  - Virulence Factors/*genetics
EDAT- 2011/05/04 06:00
MHDA- 2011/08/11 06:00
CRDT- 2011/05/04 06:00
PHST- 2010/05/19 00:00 [received]
PHST- 2010/10/02 00:00 [accepted]
PHST- 2010/09/24 00:00 [revised]
PHST- 2011/05/04 06:00 [entrez]
PHST- 2011/05/04 06:00 [pubmed]
PHST- 2011/08/11 06:00 [medline]
AID - 10.3855/jidc.1206 [doi]
PST - epublish
SO  - J Infect Dev Ctries. 2011 Apr 26;5(4):248-54. doi: 10.3855/jidc.1206.