PMID- 21537415
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 2233-6087 (Electronic)
IS  - 2233-6079 (Print)
IS  - 2233-6079 (Linking)
VI  - 35
IP  - 1
DP  - 2011 Feb
TI  - Decreased Expression and Induced Nucleocytoplasmic Translocation of Pancreatic 
      and Duodenal Homeobox 1 in INS-1 Cells Exposed to High Glucose and Palmitate.
PG  - 65-71
LID - 10.4093/dmj.2011.35.1.65 [doi]
AB  - BACKGROUND: Type 2 diabetes mellitus (T2DM) is often accompanied by increased 
      levels of circulating fatty acid. Elevations in fatty acids and glucose for 
      prolonged periods of time have been suggested to cause progressive dysfunction or 
      apoptosis of pancreatic beta cells in T2DM. However, the precise mechanism of 
      this adverse effect is not well understood. METHODS: INS-1 rat-derived 
      insulin-secreting cells were exposed to 30 mM glucose and 0.25 mM palmitate for 
      48 hours. RESULTS: The production of reactive oxygen species increased 
      significantly. Pancreatic and duodenal homeobox 1 (Pdx1) expression was 
      down-regulated, as assessed by reverse transcription-polymerase chain reaction 
      and Western blot analyses. The promoter activities of insulin and Pdx1 were also 
      diminished. Of note, there was nucleocytoplasmic translocation of Pdx1, which was 
      partially prevented by treatment with an antioxidant, N-acetyl-L-cysteine. 
      CONCLUSION: Our data suggest that prolonged exposure of beta cells to elevated 
      levels of glucose and palmitate negatively affects Pdx1 expression via oxidative 
      stress.
FAU - Ryu, Gyeong Ryul
AU  - Ryu GR
AD  - Department of Internal Medicine, College of Medicine, The Catholic University of 
      Korea, Seoul, Korea.
FAU - Yoo, Jun Mo
AU  - Yoo JM
FAU - Lee, Esder
AU  - Lee E
FAU - Ko, Seung-Hyun
AU  - Ko SH
FAU - Ahn, Yu-Bae
AU  - Ahn YB
FAU - Song, Ki-Ho
AU  - Song KH
LA  - eng
PT  - Journal Article
DEP - 20110228
PL  - Korea (South)
TA  - Diabetes Metab J
JT  - Diabetes & metabolism journal
JID - 101556588
PMC - PMC3080567
OTO - NOTNLM
OT  - Fatty acid
OT  - High glucose
OT  - Insulin
OT  - Oxidative stress
OT  - Palmitate
OT  - Pancreatic beta cell
OT  - Pdx1
EDAT- 2011/05/04 06:00
MHDA- 2011/05/04 06:01
PMCR- 2011/02/01
CRDT- 2011/05/04 06:00
PHST- 2010/05/26 00:00 [received]
PHST- 2010/09/28 00:00 [accepted]
PHST- 2011/05/04 06:00 [entrez]
PHST- 2011/05/04 06:00 [pubmed]
PHST- 2011/05/04 06:01 [medline]
PHST- 2011/02/01 00:00 [pmc-release]
AID - 10.4093/dmj.2011.35.1.65 [doi]
PST - ppublish
SO  - Diabetes Metab J. 2011 Feb;35(1):65-71. doi: 10.4093/dmj.2011.35.1.65. Epub 2011 
      Feb 28.