PMID- 21538000
OWN - NLM
STAT- MEDLINE
DCOM- 20110819
LR  - 20220410
IS  - 1613-2246 (Electronic)
IS  - 0021-5155 (Linking)
VI  - 55
IP  - 3
DP  - 2011 May
TI  - Expression profiles of cytokines and chemokines in vitreous fluid in diabetic 
      retinopathy and central retinal vein occlusion.
PG  - 256-263
LID - 10.1007/s10384-011-0004-8 [doi]
AB  - PURPOSE: The involvement of cytokines and chemokines in vitreous fluid is 
      important in the development and progression of diabetic retinopathy (DR) and 
      central retinal vein occlusion (CRVO). In this study, the concentrations of 
      cytokines and chemokines in the vitreous fluid of eyes with DR and CRVO were 
      measured and compared. METHODS: We studied 76 eyes with proliferative DR and 
      diabetic macular edema (DR group), 10 eyes with CRVO (CRVO group), and 23 eyes 
      with an epiretinal membrane and macular hole (control group), among a series of 
      160 eyes from which vitreous fluid samples were collected during vitrectomy. The 
      vitreous fluid samples were collected by suction with a vitreous cutter at the 
      initial stage of vitrectomy. Twenty-seven different cytokines and chemokines were 
      measured simultaneously using an array system (Bio-Plex((R))) with beads combined 
      with antibodies (Bio-Rad), as follows: interleukin (IL)-1beta, IL-1 receptor 
      agonist, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, 
      IL-17, eotaxin, basic fibroblast growth factor, granulocyte colony-stimulating 
      factor (G-CSF), granulocyte/macrophage colony-stimulating factor (GM-CSF), 
      interferon (IFN)-gamma, interferon-inducible 10-kDa protein (IP-10), 
      monocytochemotactic protein-1 (MCP-1), macrophage inflammatory protein-1 alpha 
      (MIP-1alpha), MIP-1beta, platelet-derived growth factor (PDGF)-BB, regulated upon 
      activation, normal T cell expressed and secreted, tumor necrosis factor alpha 
      (TNF-alpha), and vascular endothelial growth factor (VEGF). RESULTS: Compared to the 
      control group, the levels of IL-6, IL-8, IL-10, IL-13, IP-10, MCP-1, MIP-1beta, PDGF 
      and VEGF in the vitreous fluid were significantly higher in the DR group, while 
      the levels of IL-1beta, IL-2, IL-5, IL-8, IL-9, IL-10, IL-12, IL-13, eotaxin, G-CSF, 
      IFN-gamma, IP-10, MCP-1, MIP-1beta, TNF-alpha and VEGF were significantly higher in the CRVO 
      group. Compared to the DR group, IL-2, IL-9, IL-12, MCP-1 and IFN-gamma were 
      significantly elevated in the CRVO group. Multivariate regression analysis 
      revealed that among 6 factors correlated to VEGF in the DR group, IL-10 and IL-13 
      were more positively correlated and PDGF was most inversely correlated to VEGF. 
      CONCLUSION: In addition to inflammatory cytokines and neurotrophic factors such 
      as VEGF, anti-inflammatory cytokines such as IL-10 and IL-13 may be involved more 
      in the pathogenesis of DR and CRVO than in other diseases; cytokines and 
      chemokines may also be correlated to VEGF in the vitreous fluid. It is also 
      suggested that the inflammatory reaction may be more activate in CRVO than in DR.
FAU - Suzuki, Yukihiko
AU  - Suzuki Y
AD  - Department of Ophthalmology, Hirosaki University Graduate School of Medicine, 5 
      Zaifu, Hirosaki, Aomori, 036-8562, Japan. yukihiko@cc.hirosaki-u.ac.jp.
FAU - Nakazawa, Mitsuru
AU  - Nakazawa M
AD  - Department of Ophthalmology, Hirosaki University Graduate School of Medicine, 5 
      Zaifu, Hirosaki, Aomori, 036-8562, Japan.
FAU - Suzuki, Kaori
AU  - Suzuki K
AD  - Department of Ophthalmology, Hirosaki University Graduate School of Medicine, 5 
      Zaifu, Hirosaki, Aomori, 036-8562, Japan.
FAU - Yamazaki, Hitoshi
AU  - Yamazaki H
AD  - Department of Ophthalmology, Hirosaki University Graduate School of Medicine, 5 
      Zaifu, Hirosaki, Aomori, 036-8562, Japan.
FAU - Miyagawa, Yasuhiro
AU  - Miyagawa Y
AD  - Department of Ophthalmology, Hirosaki University Graduate School of Medicine, 5 
      Zaifu, Hirosaki, Aomori, 036-8562, Japan.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20110503
PL  - Japan
TA  - Jpn J Ophthalmol
JT  - Japanese journal of ophthalmology
JID - 0044652
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
SB  - IM
MH  - Aged
MH  - Chemokines/metabolism
MH  - Cytokines/*metabolism
MH  - Diabetic Retinopathy/*metabolism
MH  - Epiretinal Membrane/metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Retinal Perforations/metabolism
MH  - Retinal Vein Occlusion/*metabolism
MH  - Visual Acuity
MH  - Vitrectomy
MH  - Vitreous Body/*metabolism
EDAT- 2011/05/04 06:00
MHDA- 2011/08/20 06:00
CRDT- 2011/05/04 06:00
PHST- 2010/02/05 00:00 [received]
PHST- 2010/10/21 00:00 [accepted]
PHST- 2011/05/04 06:00 [entrez]
PHST- 2011/05/04 06:00 [pubmed]
PHST- 2011/08/20 06:00 [medline]
AID - 10.1007/s10384-011-0004-8 [pii]
AID - 10.1007/s10384-011-0004-8 [doi]
PST - ppublish
SO  - Jpn J Ophthalmol. 2011 May;55(3):256-263. doi: 10.1007/s10384-011-0004-8. Epub 
      2011 May 3.