PMID- 21538974
OWN - NLM
STAT- MEDLINE
DCOM- 20111103
LR  - 20110606
IS  - 1520-6033 (Electronic)
IS  - 1520-6033 (Linking)
VI  - 27
IP  - 3
DP  - 2011 May-Jun
TI  - Development and implementation of a perfusion-based high cell density cell 
      banking process.
PG  - 824-9
LID - 10.1002/btpr.599 [doi]
AB  - A perfusion-based high cell density (HD) cell banking process has been developed 
      that offers substantial advantages in time savings and simplification of upstream 
      unit operations. HD cell banking provides the means to reduce the time required 
      for culture inoculum expansion and scale-up by eliminating the need for multiple 
      small to intermediate scale shake flask-based operations saving up to 9 days of 
      operation during large-scale inoculum expansion. HD perfusion cultures were 
      developed and optimized in a disposable Wave bioreactor system. Through 
      optimization of perfusion rate, rocking speed and aeration rate, the perfusion 
      system supported peak cell densities of >20 x 10(6) cells/mL while maintaining 
      high cell viability (>/= 90%). The cells were frozen at HD (90-100 x 10(6) viable 
      cells/mL) in 5-mL CryoTube vials. HD cell banks were demonstrated to enable 
      direct inoculation of culture into a Wave bioreactor in the inoculum expansion 
      train thus eliminating the need for intermediate shake flask expansion unit 
      operations. The simplicity of the disposable perfusion system and high quality of 
      the cell banks resulted in the successful implementation in a 2000 L scale 
      manufacturing facility.
CI  - Copyright (c) 2011 American Institute of Chemical Engineers (AIChE).
FAU - Tao, Yiwen
AU  - Tao Y
AD  - Cell Culture Development, Biogen Idec Inc, San Diego, CA 92122, USA.
FAU - Shih, Jennifer
AU  - Shih J
FAU - Sinacore, Marty
AU  - Sinacore M
FAU - Ryll, Thomas
AU  - Ryll T
FAU - Yusuf-Makagiansar, Helena
AU  - Yusuf-Makagiansar H
LA  - eng
PT  - Journal Article
DEP - 20110427
PL  - United States
TA  - Biotechnol Prog
JT  - Biotechnology progress
JID - 8506292
SB  - IM
MH  - Bacteria/*cytology/growth & development
MH  - Bioreactors/*microbiology
MH  - *Cell Proliferation
MH  - Cryopreservation/methods
MH  - Equipment Design
MH  - *Microbial Viability
MH  - Perfusion/*methods
MH  - Quality Control
EDAT- 2011/05/04 06:00
MHDA- 2011/11/04 06:00
CRDT- 2011/05/04 06:00
PHST- 2010/11/09 00:00 [received]
PHST- 2011/02/01 00:00 [revised]
PHST- 2011/05/04 06:00 [entrez]
PHST- 2011/05/04 06:00 [pubmed]
PHST- 2011/11/04 06:00 [medline]
AID - 10.1002/btpr.599 [doi]
PST - ppublish
SO  - Biotechnol Prog. 2011 May-Jun;27(3):824-9. doi: 10.1002/btpr.599. Epub 2011 Apr 
      27.