PMID- 21538992
OWN - NLM
STAT- MEDLINE
DCOM- 20110926
LR  - 20131121
IS  - 1615-9314 (Electronic)
IS  - 1615-9306 (Linking)
VI  - 34
IP  - 12
DP  - 2011 Jun
TI  - Online coupling of molecularly imprinted solid-phase extraction to HPLC for 
      determination of trace tetracycline antibiotic residues in egg samples.
PG  - 1469-76
LID - 10.1002/jssc.201100030 [doi]
AB  - An automated system has been developed for the determination of trace 
      tetracycline antibiotics (TCs) in egg samples, based on online molecularly 
      imprinted solid-phase extraction (MISPE) coupling with high-performance liquid 
      chromatography (HPLC). Oxytetracycline and chlortetracycline were chosen as mixed 
      templates to synthesize highly selective molecularly imprinted polymers for 
      online extraction. Under the optimal online MISPE-HPLC condition, 10 mL egg 
      samples were injected into the MISPE column and then the matrix was washed out. 
      By rotating the switching valve, TCs were transferred to the analytical column 
      and then separated by HPLC. Because sample pretreatment and chromatographic 
      separation were carried out simultaneously, the whole analytical time (18 min) 
      was significantly shortened compared with conventional offline techniques. The 
      detection limits ranged from 0.8 to 1.3 ng/g. The enhancement factors were in the 
      range of 159-410. The spiked recoveries of TCs in real egg samples ranged from 
      91.6 to 107.6% and the relative standard deviations (RSDs) were not higher than 
      4.0%.
CI  - Copyright (c) 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Jing, Tao
AU  - Jing T
AD  - MOE Key Laboratory of Environment and Health, Institute of Environmental 
      Medicine, School of Public Health, Tongji Medical College, Huazhong University of 
      Science and Technology, Wuhan, Hubei, PR China.
FAU - Niu, Jiwei
AU  - Niu J
FAU - Xia, Huan
AU  - Xia H
FAU - Dai, Qing
AU  - Dai Q
FAU - Zheng, Hongyan
AU  - Zheng H
FAU - Hao, Qiaolin
AU  - Hao Q
FAU - Mei, Surong
AU  - Mei S
FAU - Zhou, Yikai
AU  - Zhou Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110503
PL  - Germany
TA  - J Sep Sci
JT  - Journal of separation science
JID - 101088554
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Polymers)
RN  - F8VB5M810T (Tetracycline)
SB  - IM
MH  - Adsorption
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis/isolation & purification
MH  - Chickens
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drug Residues/*analysis/isolation & purification
MH  - Eggs/*analysis
MH  - Food Contamination/*analysis
MH  - Molecular Imprinting
MH  - Polymers/chemistry
MH  - Solid Phase Extraction/instrumentation/*methods
MH  - Tetracycline/*analysis/isolation & purification
EDAT- 2011/05/04 06:00
MHDA- 2011/09/29 06:00
CRDT- 2011/05/04 06:00
PHST- 2011/01/11 00:00 [received]
PHST- 2011/03/23 00:00 [revised]
PHST- 2011/03/23 00:00 [accepted]
PHST- 2011/05/04 06:00 [entrez]
PHST- 2011/05/04 06:00 [pubmed]
PHST- 2011/09/29 06:00 [medline]
AID - 10.1002/jssc.201100030 [doi]
PST - ppublish
SO  - J Sep Sci. 2011 Jun;34(12):1469-76. doi: 10.1002/jssc.201100030. Epub 2011 May 3.