PMID- 21539494
OWN - NLM
STAT- MEDLINE
DCOM- 20120202
LR  - 20110826
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Linking)
VI  - 17
IP  - 17-18
DP  - 2011 Sep
TI  - Fate of bone marrow stromal cells in a syngenic model of bone formation.
PG  - 2267-78
LID - 10.1089/ten.TEA.2010.0461 [doi]
AB  - Bone marrow stromal cells (BMSCs) have been demonstrated to induce bone formation 
      when associated to osteoconductive biomaterials and implanted in vivo. 
      Nevertheless, their role in bone reconstruction is not fully understood and rare 
      studies have been conducted to follow their destiny after implantation in 
      syngenic models. The aim of the present work was to use sensitive and 
      quantitative methods to track donor and recipient cells after implantation of 
      BMSCs in a syngenic model of ectopic bone formation. Using polymerase chain 
      reaction (PCR) amplification of the Sex determining Region Y (Sry) gene and in 
      situ hybridization of the Y chromosome in parallel to histological analysis, we 
      have quantified within the implants the survival of the donor cells and the 
      colonization by the recipient cells. The putative migration of the BMSCs in 
      peripheral organs was also analyzed. We show here that grafted cells do not 
      survive more than 3 weeks after implantation and might migrate in peripheral 
      lymphoid organs. These cells are responsible for the attraction of host cells 
      within the implants, leading to the centripetal colonization of the biomaterial 
      by new bone.
FAU - Boukhechba, Florian
AU  - Boukhechba F
AD  - GEPITOS, Universite Nice Sophia-Antipolis, CNRS, UFR de Medecine, Nice, France.
FAU - Balaguer, Thierry
AU  - Balaguer T
FAU - Bouvet-Gerbettaz, Sebastien
AU  - Bouvet-Gerbettaz S
FAU - Michiels, Jean-Francois
AU  - Michiels JF
FAU - Bouler, Jean-Michel
AU  - Bouler JM
FAU - Carle, Georges F
AU  - Carle GF
FAU - Scimeca, Jean-Claude
AU  - Scimeca JC
FAU - Rochet, Nathalie
AU  - Rochet N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110614
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (Sex-Determining Region Y Protein)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Cell Movement/genetics/physiology
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Osteogenesis/physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sex-Determining Region Y Protein/genetics
MH  - Stromal Cells/*cytology/metabolism
MH  - Y Chromosome/genetics
EDAT- 2011/05/05 06:00
MHDA- 2012/02/03 06:00
CRDT- 2011/05/05 06:00
PHST- 2011/05/05 06:00 [entrez]
PHST- 2011/05/05 06:00 [pubmed]
PHST- 2012/02/03 06:00 [medline]
AID - 10.1089/ten.TEA.2010.0461 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2011 Sep;17(17-18):2267-78. doi: 10.1089/ten.TEA.2010.0461. 
      Epub 2011 Jun 14.