PMID- 2153965
OWN - NLM
STAT- MEDLINE
DCOM- 19900306
LR  - 20210409
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 87
IP  - 2
DP  - 1990 Jan
TI  - Isolation of a new herpesvirus from human CD4+ T cells.
PG  - 748-52
AB  - A new human herpesvirus has been isolated from CD4+ T cells purified from 
      peripheral blood mononuclear cells of a healthy individual (RK), following 
      incubation of the cells under conditions promoting T-cell activation. The virus 
      could not be recovered from nonactivated cells. Cultures of lymphocytes infected 
      with the RK virus exhibited a cytopathic effect, and electron microscopic 
      analyses revealed a characteristic herpesvirus structure. RK virus DNA did not 
      hybridize with large probes derived from herpes simplex virus, Epstein-Barr 
      virus, varicella-zoster virus, and human cytomegalovirus. The genetic relatedness 
      of the RK virus to the recently identified T-lymphotropic human herpesvirus 6 
      (HHV-6) was investigated by restriction enzyme analyses using 21 different 
      enzymes and by blot hybridization analyses using 11 probes derived from two 
      strains of HHV-6 (Z29 and U1102). Whereas the two HHV-6 strains exhibited only 
      limited restriction enzyme polymorphism, cleavage of the RK virus DNA yielded 
      distinct patterns. Of the 11 HHV-6 DNA probes tested, only 6 cross-hybridized 
      with DNA fragments derived from the RK virus. Taken together, the maximal 
      homology amounted to 31 kilobases of the 75 kilobases tested. We conclude that 
      the RK virus is distinct from previously characterized human herpesviruses. We 
      propose to designate it as the prototype of a new herpesvirus, the seventh human 
      herpesvirus identified to date.
FAU - Frenkel, N
AU  - Frenkel N
AD  - Laboratory of Viral Diseases, National Institute of Allergy and Infectious 
      Diseases/Twinbrook II, National Institutes of Health, Rockville, MD 20852.
FAU - Schirmer, E C
AU  - Schirmer EC
FAU - Wyatt, L S
AU  - Wyatt LS
FAU - Katsafanas, G
AU  - Katsafanas G
FAU - Roffman, E
AU  - Roffman E
FAU - Danovich, R M
AU  - Danovich RM
FAU - June, C H
AU  - June CH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (CD4 Antigens)
RN  - 0 (DNA, Viral)
SB  - IM
EIN - Proc Natl Acad Sci U S A 1990 Oct;87(19):7797
MH  - CD4 Antigens/*analysis
MH  - DNA, Viral/genetics/isolation & purification
MH  - Flow Cytometry
MH  - Genes, Viral
MH  - Humans
MH  - Lymphocyte Activation
MH  - Microscopy, Electron
MH  - Nucleic Acid Hybridization
MH  - Restriction Mapping
MH  - Sequence Homology, Nucleic Acid
MH  - Simplexvirus/*isolation & purification/ultrastructure
MH  - T-Lymphocytes/cytology/immunology/*microbiology
PMC - PMC53343
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
PMCR- 1990/07/01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
PHST- 1990/07/01 00:00 [pmc-release]
AID - 10.1073/pnas.87.2.748 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1990 Jan;87(2):748-52. doi: 10.1073/pnas.87.2.748.