PMID- 21540110
OWN - NLM
STAT- MEDLINE
DCOM- 20120411
LR  - 20110929
IS  - 1873-3441 (Electronic)
IS  - 0939-6411 (Linking)
VI  - 79
IP  - 2
DP  - 2011 Oct
TI  - ToF-SIMS analysis of ocular tissues reveals biochemical differentiation and drug 
      distribution.
PG  - 328-33
LID - 10.1016/j.ejpb.2011.04.006 [doi]
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to obtain mass 
      spectra from three ocular tissues, the lens, the vitreous and the retina. All 
      three tissues were extracted from control ovine eyes and ovine eyes treated with 
      model drug. To identify variations in surface biochemistry of each ocular tissue, 
      principal component analysis (PCA) was applied to ToF-SIMS data. Interesting 
      physiological differences in Na(+) and K(+) distribution were shown across the 
      three tissue types, with other elements including Ca(2+) and Fe(2+) distribution 
      also detected. In addition to the identification of small molecules and smaller 
      molecular fragments, larger molecules such as phosphocholine were also detected. 
      The ToF-SIMS data were also used to identify the presence of a model drug 
      compound (amitriptyline--chosen as a generic drug structure) within all three 
      ocular tissues, with model drug detected predominantly across the vitreous tissue 
      samples. This study demonstrates that PCA can be successfully applied to ToF-SIMS 
      data from different ocular tissues and highlights the potential of coupling 
      multivariate statistics with surface analytical techniques to gain a greater 
      understanding of the biochemical composition of tissues and the distribution of 
      pharmaceutically active small molecules within these tissues.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Mains, Jenifer
AU  - Mains J
AD  - Strathclyde Institute of Pharmacy and Biomedical Sciences, University of 
      Strathclyde, Glasgow, Scotland, United Kingdom.
FAU - Wilson, Clive
AU  - Wilson C
FAU - Urquhart, Andrew
AU  - Urquhart A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110421
PL  - Netherlands
TA  - Eur J Pharm Biopharm
JT  - European journal of pharmaceutics and biopharmaceutics : official journal of 
      Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
JID - 9109778
SB  - IM
MH  - Animals
MH  - Eye/*chemistry/*metabolism
MH  - Lens, Crystalline/chemistry/metabolism
MH  - Multivariate Analysis
MH  - Pharmacokinetics
MH  - Principal Component Analysis/*methods
MH  - Retina/chemistry/metabolism
MH  - Sheep
MH  - Spectrometry, Mass, Secondary Ion/*methods
MH  - Tissue Distribution
MH  - Vitreous Body/chemistry/metabolism
EDAT- 2011/05/05 06:00
MHDA- 2012/04/12 06:00
CRDT- 2011/05/05 06:00
PHST- 2011/01/17 00:00 [received]
PHST- 2011/03/03 00:00 [revised]
PHST- 2011/04/12 00:00 [accepted]
PHST- 2011/05/05 06:00 [entrez]
PHST- 2011/05/05 06:00 [pubmed]
PHST- 2012/04/12 06:00 [medline]
AID - S0939-6411(11)00147-0 [pii]
AID - 10.1016/j.ejpb.2011.04.006 [doi]
PST - ppublish
SO  - Eur J Pharm Biopharm. 2011 Oct;79(2):328-33. doi: 10.1016/j.ejpb.2011.04.006. 
      Epub 2011 Apr 21.