PMID- 21540305
OWN - NLM
STAT- MEDLINE
DCOM- 20120620
LR  - 20201226
IS  - 1399-3003 (Electronic)
IS  - 0903-1936 (Linking)
VI  - 38
IP  - 5
DP  - 2011 Nov
TI  - Recognition of pleural mesothelioma by mucin-1(950-958)/human leukocyte antigen 
      A*0201-specific CD8+ T-cells.
PG  - 1117-26
LID - 10.1183/09031936.00160210 [doi]
AB  - Recent clinical investigations have demonstrated that T-cell-based immunotherapy 
      of malignant pleural mesothelioma (MPM) could represent an alternative to the 
      other therapeutic strategies. However, its development suffers from the lack of 
      identified tumour antigenic targets. Mucin (MUC)1, which is expressed and 
      recognised by cytotoxic T-cells in numerous cancer types, has not been 
      investigated as a potential immune target in MPM. Thus, the objective of this 
      study was to analyse MUC1 expression by MPM cells and to determine whether this 
      antigen can be the target of cytotoxic CD8+ T-cells (cytotoxic T-lymphocytes 
      (CTLs)). We first evaluated the expression and glycosylation of MUC1 by MPM cell 
      lines using different MUC1-specific monoclonal antibodies. We then obtained a CTL 
      clone specific for a MUC1 peptide (residues 950-958) presented by human leukocyte 
      antigen (HLA)-A*0201 and studied its interferon-gamma and cytotoxic response to MPM 
      cell lines. We found that all MPM cell lines expressed MUC1 protein at the cell 
      surface with different glycosylation profiles. We also observed that HLA-A*0201+ 
      MPM cell lines are recognised and lysed by a HLA-A*0201/MUC1(950-958)-specific 
      CTL clone independently of the MUC1 glycosylation profile. Thus, MUC1 expression 
      and antigen presentation by MPM cells may represent an attractive target for 
      immunotherapeutic treatment of MPM despite its hyperglycosylated profile.
FAU - Roulois, D
AU  - Roulois D
AD  - INSERM, U892 Institut de Recherche Therapeutique, Universite de Nantes, Nantes 
      Cedex 1, France.
FAU - Vignard, V
AU  - Vignard V
FAU - Gueugnon, F
AU  - Gueugnon F
FAU - Labarriere, N
AU  - Labarriere N
FAU - Gregoire, M
AU  - Gregoire M
FAU - Fonteneau, J-F
AU  - Fonteneau JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110503
PL  - England
TA  - Eur Respir J
JT  - The European respiratory journal
JID - 8803460
RN  - 0 (HLA-A*02:01 antigen)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Mucin-1)
SB  - IM
MH  - Antigen Presentation/immunology
MH  - CD8-Positive T-Lymphocytes/*immunology/metabolism
MH  - Cell Line, Tumor
MH  - Clone Cells
MH  - Glycosylation
MH  - HLA-A2 Antigen/immunology/*metabolism
MH  - Humans
MH  - Mesothelioma/*immunology
MH  - Mucin-1/immunology/*metabolism
MH  - Pleural Neoplasms/*immunology/metabolism
MH  - T-Lymphocytes, Cytotoxic/*immunology/metabolism
EDAT- 2011/05/05 06:00
MHDA- 2012/06/21 06:00
CRDT- 2011/05/05 06:00
PHST- 2011/05/05 06:00 [entrez]
PHST- 2011/05/05 06:00 [pubmed]
PHST- 2012/06/21 06:00 [medline]
AID - 09031936.00160210 [pii]
AID - 10.1183/09031936.00160210 [doi]
PST - ppublish
SO  - Eur Respir J. 2011 Nov;38(5):1117-26. doi: 10.1183/09031936.00160210. Epub 2011 
      May 3.