PMID- 21543119
OWN - NLM
STAT- MEDLINE
DCOM- 20111214
LR  - 20110711
IS  - 1878-1667 (Electronic)
IS  - 0147-9571 (Linking)
VI  - 34
IP  - 4
DP  - 2011 Jul
TI  - Systemic and mucosal immunity induced by attenuated Salmonella enterica serovar 
      Typhimurium expressing ORF7 of porcine reproductive and respiratory syndrome 
      virus.
PG  - 335-45
LID - 10.1016/j.cimid.2011.04.001 [doi]
AB  - Oral administration of attenuated Salmonella vaccine may provide valuable 
      advantages such as low cost, easy preparation, and safety. Attenuated Salmonella 
      vaccines also serve as carriers of foreign antigens and immunomodulatory 
      cytokines. Presently, an attenuated Salmonella enterica serovar Typhimurium 
      strain was used as a carrier for open reading frame 7 (ORF7) protein of porcine 
      reproductive and respiratory syndrome virus (PRRSV), a swine pathogen of 
      significant global economic importance. Initially, an attenuated S. enterica 
      serovar Typhimurium expressing ORF7 gene derived from PRRSV Korean isolate was 
      constructed. Following oral administration of a single dose of the attenuated 
      Salmonella vaccine expressing PRRSV ORF7, humoral and cell-mediated immune 
      responses specific for ORF7 were induced at both systemic and mucosal sites 
      including spleen, mesenteric lymph node, Peyer's patch, and laminar propria, as 
      evaluated by determining serum ORF7-specific IgG and mucosal IgA responses, as 
      well as Th1- and Th2-type cytokine production from antigen-stimulated T cells. 
      The induced humoral responses were sustained for at least 12weeks 
      post-immunization. In particular, the immunized mice displayed immune responses 
      to both the foreign ORF7 antigen and Salmonella itself. The results indicate the 
      value of attenuated S. enterica serovar Typhimurium as an oral carrier of PRRSV 
      antigenic proteins to induce effective systemic and mucosal immunity.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Han, Young Woo
AU  - Han YW
AD  - College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk 
      National University, Jeonju, Republic of Korea.
FAU - Kim, Seong Bum
AU  - Kim SB
FAU - Rahman, Masudur
AU  - Rahman M
FAU - Uyangaa, Erdenebileg
AU  - Uyangaa E
FAU - Lee, Byung Min
AU  - Lee BM
FAU - Kim, Jin Hyoung
AU  - Kim JH
FAU - Park, Ki In
AU  - Park KI
FAU - Hong, Jin Tae
AU  - Hong JT
FAU - Han, Sang-Bae
AU  - Han SB
FAU - Eo, Seong Kug
AU  - Eo SK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110502
PL  - England
TA  - Comp Immunol Microbiol Infect Dis
JT  - Comparative immunology, microbiology and infectious diseases
JID - 7808924
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Antigens, Viral)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Vaccines, Attenuated)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Antigens, Bacterial
MH  - Antigens, Viral/immunology
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Female
MH  - *Immunity, Mucosal
MH  - Immunoglobulin A/analysis/biosynthesis
MH  - Immunoglobulin G/analysis/biosynthesis
MH  - Lymph Nodes/cytology/*immunology/microbiology
MH  - Lymphocyte Activation
MH  - Lymphocytes/*immunology/microbiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Open Reading Frames/genetics/immunology
MH  - Peyer's Patches/cytology/*immunology/microbiology
MH  - Porcine respiratory and reproductive syndrome 
      virus/chemistry/*genetics/immunology
MH  - Salmonella Infections, Animal/immunology/metabolism/*prevention & control
MH  - Salmonella typhimurium/growth & development/immunology
MH  - Spleen/cytology/*immunology/microbiology
MH  - Swine
MH  - Th1-Th2 Balance
MH  - *Vaccination
MH  - Vaccines, Attenuated/*administration & dosage
EDAT- 2011/05/06 06:00
MHDA- 2011/12/15 06:00
CRDT- 2011/05/06 06:00
PHST- 2010/12/12 00:00 [received]
PHST- 2011/03/22 00:00 [revised]
PHST- 2011/04/04 00:00 [accepted]
PHST- 2011/05/06 06:00 [entrez]
PHST- 2011/05/06 06:00 [pubmed]
PHST- 2011/12/15 06:00 [medline]
AID - S0147-9571(11)00027-0 [pii]
AID - 10.1016/j.cimid.2011.04.001 [doi]
PST - ppublish
SO  - Comp Immunol Microbiol Infect Dis. 2011 Jul;34(4):335-45. doi: 
      10.1016/j.cimid.2011.04.001. Epub 2011 May 2.