PMID- 21543205
OWN - NLM
STAT- MEDLINE
DCOM- 20120529
LR  - 20181201
IS  - 1873-4847 (Electronic)
IS  - 0955-2863 (Linking)
VI  - 23
IP  - 4
DP  - 2012 Apr
TI  - Purple corn anthocyanins dampened high-glucose-induced mesangial fibrosis and 
      inflammation: possible renoprotective role in diabetic nephropathy.
PG  - 320-31
LID - 10.1016/j.jnutbio.2010.12.008 [doi]
AB  - Purple corn has been classified as a functional food rich in anthocyanins 
      possessing potential disease-preventive properties. This study examined whether 
      purple corn anthocyanins (PCA) mainly comprised cyanidin 3-glucoside and 
      cyanidin-3-(6''-malonylglucoside) can attenuate high-glucose (HG)-promoted 
      mesangial cell (MC) proliferation and matrix accumulation, major features of 
      diabetic glomerulosclerosis. Human renal MC were cultured for 3 days in media 
      containing 5.5 mM glucose plus 27.5 mM mannitol as osmotic controls or media 
      containing 33 mM glucose in the absence and presence of 1-20 mug/ml PCA. The HG 
      exposure of MC caused substantial increases in connective tissue growth factor 
      (CTGF) expression and collagen IV secretion with mesangial hyperplasia, which 
      were repealed by adding PCA. PCA boosted HG-plummeted membrane type-1 matrix 
      metalloproteinase expression and dampened HG-elevated tissue inhibitor of matrix 
      metalloproteinase-2 expression through disturbing transforming growth factor beta 
      (TGF-beta)-SMAD signaling, facilitating extracellular matrix degradation. This study 
      further revealed that PCA ameliorated HG-inflamed mesangial inflammation 
      accompanying induction of intracellular cell adhesion molecule-1 and monocyte 
      chemoattractant protein-1 (MCP-1) responsible for CTGF expression. The induction 
      of intracellular cell adhesion molecule-1 and MCP-1 was mediated via TGF-beta 
      signaling, which was suppressed by PCA. In addition, the HG-promoted CTGF 
      expression entailed nuclear factor kappaB (NF-kappaB) signaling involved in MCP-1 
      transcription. The HG-TGF-beta induction was blocked in the presence of a NF-kappaB 
      inhibitor, and the nuclear NF-kappaB translocation was blunted by a TGF-beta receptor 1 
      inhibitor. PCA dampened NF-kappaB translocation in HG-exposed MC. These results 
      demonstrate that there was a crosstalk between TGF-beta-SMAD and NF-kappaB pathways in 
      the diabetes-associated mesangial fibrosis and inflammation, which appeared to be 
      severed by PCA.
CI  - Copyright A(c) 2012 Elsevier Inc. All rights reserved.
FAU - Li, Jing
AU  - Li J
AD  - Department of Food and Nutrition and the Regional Research Universities 
      Program/Medical & Bio-Materials Research Center, Hallym University, Chuncheon 
      200-702, Korea.
FAU - Lim, Soon Sung
AU  - Lim SS
FAU - Lee, Jae-Yong
AU  - Lee JY
FAU - Kim, Jin-Kyu
AU  - Kim JK
FAU - Kang, Sang-Wook
AU  - Kang SW
FAU - Kim, Jung-Lye
AU  - Kim JL
FAU - Kang, Young-Hee
AU  - Kang YH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110502
PL  - United States
TA  - J Nutr Biochem
JT  - The Journal of nutritional biochemistry
JID - 9010081
RN  - 0 (Anthocyanins)
RN  - 0 (Collagen Type IV)
RN  - 0 (NF-kappa B)
RN  - 0 (Transforming Growth Factor beta)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Anthocyanins/*pharmacology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Collagen Type IV/drug effects/metabolism
MH  - Connective Tissue Growth Factor/metabolism
MH  - Diabetic Nephropathies/*prevention & control
MH  - Fibrosis
MH  - Glomerular Mesangium/drug effects/pathology
MH  - Glucose/administration & dosage/metabolism
MH  - Humans
MH  - Inflammation/*chemically induced/metabolism/pathology
MH  - Intercellular Adhesion Molecule-1/genetics/metabolism
MH  - Matrix Metalloproteinase 2/genetics/metabolism
MH  - Mesangial Cells/*drug effects/pathology
MH  - NF-kappa B/genetics/metabolism
MH  - Signal Transduction
MH  - Transforming Growth Factor beta/genetics/metabolism
MH  - Zea mays/*chemistry
EDAT- 2011/05/06 06:00
MHDA- 2012/05/30 06:00
CRDT- 2011/05/06 06:00
PHST- 2010/07/14 00:00 [received]
PHST- 2010/11/29 00:00 [revised]
PHST- 2010/12/09 00:00 [accepted]
PHST- 2011/05/06 06:00 [entrez]
PHST- 2011/05/06 06:00 [pubmed]
PHST- 2012/05/30 06:00 [medline]
AID - S0955-2863(11)00037-4 [pii]
AID - 10.1016/j.jnutbio.2010.12.008 [doi]
PST - ppublish
SO  - J Nutr Biochem. 2012 Apr;23(4):320-31. doi: 10.1016/j.jnutbio.2010.12.008. Epub 
      2011 May 2.