PMID- 21543472
OWN - NLM
STAT- MEDLINE
DCOM- 20110915
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 85
IP  - 14
DP  - 2011 Jul
TI  - Role of CD4+ and CD8+ T-cell responses against JC virus in the outcome of 
      patients with progressive multifocal leukoencephalopathy (PML) and PML with 
      immune reconstitution inflammatory syndrome.
PG  - 7256-63
LID - 10.1128/JVI.02506-10 [doi]
AB  - Progressive multifocal leukoencephalopathy (PML) is a severe demyelinating 
      disease of the brain caused by JC virus (JCV). To assess the role of CD4(+) and 
      CD8(+) T-cells against JCV in the clinical outcome of PML and PML in the setting 
      of immune reconstitution inflammatory syndrome (IRIS), we tested gamma interferon 
      (IFN-gamma) response by enzyme-linked immunosorbent spot (ELISpot) and intracellular 
      cytokine staining (ICS) in 117 subjects, including 66 PML patients with different 
      clinical outcomes. Both assays were concordant and demonstrated that the cellular 
      immune response against JCV is associated with better clinical outcome. PML 
      survivors had an early CD8(+) T-cell response more frequently than PML 
      progressors (100% versus 27.3%; P = 0.001), while only a trend was observed for 
      the early CD4(+) T-cell response between these two groups (80% versus 45.5%; P = 
      0.18). Although IRIS itself was more frequent in the PML survivor group, there 
      was no difference in IFN-gamma-producing CD4(+) and CD8(+) T-cells between IRIS and 
      non-IRIS PML patients, suggesting that T-cells expressing other cytokines likely 
      have a role in the immunopathogenesis of IRIS. ELISpot and ICS assays are useful 
      prognostic markers of PML evolution and may help in the clinical management of 
      these patients.
FAU - Gheuens, Sarah
AU  - Gheuens S
AD  - Division of NeuroVirology, Beth Israel Deaconess Medical Center, E/CLS-1005, 330 
      Brookline Avenue, Boston, MA 02215, USA.
FAU - Bord, Evelyn
AU  - Bord E
FAU - Kesari, Santosh
AU  - Kesari S
FAU - Simpson, David M
AU  - Simpson DM
FAU - Gandhi, Rajesh T
AU  - Gandhi RT
FAU - Clifford, David B
AU  - Clifford DB
FAU - Berger, Joseph R
AU  - Berger JR
FAU - Ngo, Long
AU  - Ngo L
FAU - Koralnik, Igor J
AU  - Koralnik IJ
LA  - eng
GR  - T32 AI007387/AI/NIAID NIH HHS/United States
GR  - K24 NS 060950/NS/NINDS NIH HHS/United States
GR  - R56 NS041198/NS/NINDS NIH HHS/United States
GR  - R56 NS 041198/NS/NINDS NIH HHS/United States
GR  - R01 NS 047029/NS/NINDS NIH HHS/United States
GR  - UL1 RR 025758/RR/NCRR NIH HHS/United States
GR  - P30 AI060354/AI/NIAID NIH HHS/United States
GR  - T32 CA09031-32/CA/NCI NIH HHS/United States
GR  - P30 AI60354/AI/NIAID NIH HHS/United States
GR  - UL1 RR025758/RR/NCRR NIH HHS/United States
GR  - T32 CA009031/CA/NCI NIH HHS/United States
GR  - R01 NS047029/NS/NINDS NIH HHS/United States
GR  - K24 NS060950/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110504
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
SB  - IM
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Humans
MH  - Inflammation/*immunology
MH  - JC Virus/*immunology
MH  - Leukoencephalopathy, Progressive Multifocal/*immunology
PMC - PMC3126613
EDAT- 2011/05/06 06:00
MHDA- 2011/09/16 06:00
PMCR- 2012/01/01
CRDT- 2011/05/06 06:00
PHST- 2011/05/06 06:00 [entrez]
PHST- 2011/05/06 06:00 [pubmed]
PHST- 2011/09/16 06:00 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - JVI.02506-10 [pii]
AID - 2506-10 [pii]
AID - 10.1128/JVI.02506-10 [doi]
PST - ppublish
SO  - J Virol. 2011 Jul;85(14):7256-63. doi: 10.1128/JVI.02506-10. Epub 2011 May 4.