PMID- 21543500
OWN - NLM
STAT- MEDLINE
DCOM- 20110915
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 85
IP  - 14
DP  - 2011 Jul
TI  - Identification and expression analysis of herpes B virus-encoded small RNAs.
PG  - 7296-311
LID - 10.1128/JVI.00505-11 [doi]
AB  - Herpes B virus (BV) naturally infects macaque monkeys and is genetically similar 
      to herpes simplex virus (HSV). Zoonotic infection of humans can cause 
      encephalitis and if untreated has a fatality rate of  approximately 80%. The frequent use of 
      macaques in biomedical research emphasizes the need to understand the molecular 
      basis of BV pathogenesis with a view toward improving safety for those working 
      with macaques. MicroRNAs (miRNAs) are small noncoding RNAs that regulate the 
      expression of mRNAs bearing complementary target sequences and are employed by 
      viruses to control viral and host gene expression. Using deep sequencing and 
      validation by expression in transfected cells, we identified 12 novel BV-encoded 
      miRNAs expressed in lytically infected cells and 4 in latently infected 
      trigeminal ganglia (TG). Using quantitative reverse transcription-PCR (RT-qPCR), 
      we found that most of the miRNAs exhibited a high level of abundance throughout 
      infection. Further analyses showed that some miRNAs could be generated from 
      multiple transcripts with different kinetic classes, possibly explaining 
      detection throughout infection. Interestingly, miRNAs were detected at early 
      times in the absence of viral gene expression and were present in purified 
      virions. In TG, despite similar amounts of viral DNA per ganglion, it was notable 
      that the relative amount of each miRNA varied between ganglia. The majority of 
      the miRNAs are encoded by the regions that exhibit the most sequence differences 
      between BV and HSV. Additionally, there is no sequence conservation between BV- 
      and HSV-encoded miRNAs, which may be important for the differences in the human 
      diseases caused by BV and HSV.
FAU - Amen, Melanie A
AU  - Amen MA
AD  - Department of Virology and Immunology, Texas Biomedical Research Institute, P.O. 
      Box 760549, San Antonio, TX 78227, USA.
FAU - Griffiths, Anthony
AU  - Griffiths A
LA  - eng
GR  - C06 RR012087/RR/NCRR NIH HHS/United States
GR  - P51 RR013986/RR/NCRR NIH HHS/United States
GR  - R21 RR026287/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110504
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chlorocebus aethiops
MH  - DNA Primers
MH  - HeLa Cells
MH  - Herpesvirus 1, Cercopithecine/*genetics
MH  - Humans
MH  - Macaca mulatta
MH  - RNA, Viral/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Vero Cells
PMC - PMC3126578
EDAT- 2011/05/06 06:00
MHDA- 2011/09/16 06:00
PMCR- 2012/01/01
CRDT- 2011/05/06 06:00
PHST- 2011/05/06 06:00 [entrez]
PHST- 2011/05/06 06:00 [pubmed]
PHST- 2011/09/16 06:00 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - JVI.00505-11 [pii]
AID - 0505-11 [pii]
AID - 10.1128/JVI.00505-11 [doi]
PST - ppublish
SO  - J Virol. 2011 Jul;85(14):7296-311. doi: 10.1128/JVI.00505-11. Epub 2011 May 4.