PMID- 21543643
OWN - NLM
STAT- MEDLINE
DCOM- 20111007
LR  - 20121115
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 186
IP  - 12
DP  - 2011 Jun 15
TI  - Identification of IDO-positive and IDO-negative human dendritic cells after 
      activation by various proinflammatory stimuli.
PG  - 6701-9
LID - 10.4049/jimmunol.1003151 [doi]
AB  - Dendritic cells (DCs) can induce tolerance or immunity. We identified and 
      characterized an IDO-expressing and an IDO-negative human DC population after 
      stimulation by various proinflammatory stimuli. IDO expression was strongly 
      dependent on the maturation status of the cells (CD83-positive cells only). The 
      two DC subpopulations remained IDO positive and IDO negative, respectively, over 
      a time period of at least 48 h. IDO enzyme activity of human DCs was highest 
      during stimulation by strongly maturation-inducing TLR ligands such as highly 
      purified LPS (TLR4 ligand) or polyriboinosinic-polyribocytidilic acid (TLR3 
      ligand); factors of the adaptive immune system such as IFN-gamma, a mixture of 
      cytokines, and IFN-alpha had lesser stimulatory capacity for IDO induction and 
      activity. After stimulation with CD40L, IDO-positive DCs expressed significantly 
      increased levels of B7 family molecules such as CD40, CD80, CD86, ICOS ligand, as 
      well as PD-L1 (B7-H1) and PD-L2 (B7-DC) compared with the IDO-negative DC subset. 
      At the same time, the inhibitory receptors Ig-like transcripts 3 and 4 were 
      significantly downregulated on IDO-positive cells. Functionally, IDO-positive DCs 
      produced significantly more IL-1beta and IL-15 and less IL-10 and IL-6 than the 
      IDO-negative subset after CD40L stimulation. These results show that IDO 
      expression is associated with a distinctive phenotype and functional capacity in 
      mature DCs. It seems likely that the IDO-positive DC subset possesses a 
      regulatory function and might skew a T cell response toward tolerance.
FAU - Von Bubnoff, Dagmar
AU  - Von Bubnoff D
AD  - Department of Dermatology and Allergy, Friedrich-Wilhelms-University of Bonn, 
      53105 Bonn, Germany. d.bubnoff@uni-bonn.de
FAU - Scheler, Marina
AU  - Scheler M
FAU - Wilms, Helene
AU  - Wilms H
FAU - Fimmers, Rolf
AU  - Fimmers R
FAU - Bieber, Thomas
AU  - Bieber T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110504
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase)
RN  - 0 (Interleukins)
RN  - 0 (Ligands)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Cells, Cultured
MH  - Dendritic Cells/*enzymology
MH  - Humans
MH  - Immune Tolerance/immunology
MH  - Indoleamine-Pyrrole 2,3,-Dioxygenase/*analysis
MH  - Inflammation/*chemically induced
MH  - Interleukins/biosynthesis
MH  - Ligands
MH  - Phenotype
MH  - Toll-Like Receptors/agonists
EDAT- 2011/05/06 06:00
MHDA- 2011/10/08 06:00
CRDT- 2011/05/06 06:00
PHST- 2011/05/06 06:00 [entrez]
PHST- 2011/05/06 06:00 [pubmed]
PHST- 2011/10/08 06:00 [medline]
AID - jimmunol.1003151 [pii]
AID - 10.4049/jimmunol.1003151 [doi]
PST - ppublish
SO  - J Immunol. 2011 Jun 15;186(12):6701-9. doi: 10.4049/jimmunol.1003151. Epub 2011 
      May 4.