PMID- 21544801
OWN - NLM
STAT- MEDLINE
DCOM- 20111220
LR  - 20211020
IS  - 1097-0215 (Electronic)
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 129
IP  - 12
DP  - 2011 Dec 15
TI  - Functional interrelationship between the WASF3 and KISS1 metastasis-associated 
      genes in breast cancer cells.
PG  - 2825-35
LID - 10.1002/ijc.25964 [doi]
AB  - Loss of WASF3 function in breast cancer cells results in loss of invasion 
      phenotypes and reduced metastatic potential. By using oligonucleotide arrays, we 
      now demonstrate that knockdown of WASF3 leads to the upregulation of the KISS1 
      metastasis suppressor gene with concomitant reduced invasion and loss of matrix 
      metalloproteinases (MMP)-9 activity. Using a luciferase reporter, KISS1 
      transcription is significantly increased in the absence of WASF3. Knockdown of 
      KISS1 in WASF3-silenced cells resulted in the recovery of the invasion phenotype. 
      WASF3 knockdown also resulted in elevated IkappaBalpha levels in the cytoplasm and 
      reduced levels of nuclear factor-kappa-B (NF-kappaB) p65/50 subunits in the nucleus. 
      Tumor necrosis factor-alpha (TNF-alpha) has been associated with cell invasion 
      through induction of MMP-9 production via KISS1 regulation of the NF-kappaB pathway. 
      When WASF3 knockdown cells are treated with TNF-alpha, no effect is seen on invasion 
      or nuclear translocation of NF-kappaB. Thus, coordinated expression patterns of the 
      WASF3 metastasis promoter gene and the KISS1 metastasis suppressor gene appear to 
      exert their influence through inhibition of NF-kappaB signaling, which in turn 
      regulates MMP-9 production facilitating invasion.
CI  - Copyright (c) 2011 UICC.
FAU - Teng, Yong
AU  - Teng Y
AD  - School of Medicine, Medical College of Georgia, Augusta, GA, USA.
FAU - Liu, Mingyao
AU  - Liu M
FAU - Cowell, John K
AU  - Cowell JK
LA  - eng
GR  - R01 CA120510/CA/NCI NIH HHS/United States
GR  - R01 CA120510-01A1/CA/NCI NIH HHS/United States
GR  - CA120510/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110505
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (KISS1 protein, human)
RN  - 0 (Kisspeptins)
RN  - 0 (NF-kappa B)
RN  - 0 (WASF3 protein, human)
RN  - 0 (Wiskott-Aldrich Syndrome Protein Family)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Breast Neoplasms/*genetics
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Female
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Kisspeptins/*genetics/metabolism
MH  - Male
MH  - Matrix Metalloproteinase 9/metabolism
MH  - NF-kappa B/metabolism
MH  - Neoplasm Invasiveness
MH  - Neoplasm Metastasis/*genetics
MH  - Prostatic Neoplasms/metabolism
MH  - Signal Transduction/genetics
MH  - Up-Regulation
MH  - Wiskott-Aldrich Syndrome Protein Family/*genetics/metabolism
PMC - PMC3154992
MID - NIHMS275139
EDAT- 2011/05/06 06:00
MHDA- 2011/12/21 06:00
PMCR- 2012/12/15
CRDT- 2011/05/06 06:00
PHST- 2010/11/02 00:00 [received]
PHST- 2011/01/12 00:00 [accepted]
PHST- 2011/05/06 06:00 [entrez]
PHST- 2011/05/06 06:00 [pubmed]
PHST- 2011/12/21 06:00 [medline]
PHST- 2012/12/15 00:00 [pmc-release]
AID - 10.1002/ijc.25964 [doi]
PST - ppublish
SO  - Int J Cancer. 2011 Dec 15;129(12):2825-35. doi: 10.1002/ijc.25964. Epub 2011 May 
      5.