PMID- 21544902
OWN - NLM
STAT- MEDLINE
DCOM- 20111115
LR  - 20211020
IS  - 1549-4918 (Electronic)
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 29
IP  - 7
DP  - 2011 Jul
TI  - Bone marrow-derived mesenchymal stromal cells inhibit Th2-mediated allergic 
      airways inflammation in mice.
PG  - 1137-48
LID - 10.1002/stem.656 [doi]
AB  - Bone marrow-derived mesenchymal stromal cells (BMSCs) mitigate inflammation in 
      mouse models of acute lung injury. However, specific mechanisms of BMSC actions 
      on CD4 T lymphocyte-mediated inflammation in vivo remain poorly understood. 
      Limited data suggests promotion of Th2 phenotype in models of Th1-mediated 
      diseases. However, whether this might alleviate or worsen Th2-mediated diseases 
      such as allergic asthma is unknown. To ascertain the effects of systemic 
      administration of BMSCs in a mouse model of Th2-mediated allergic airways 
      inflammation, ovalbumin (OVA)-induced allergic airways inflammation was induced 
      in wild-type C57BL/6 and BALB/c mice as well as in interferon-gamma (IFNgamma) receptor 
      null mice. Effects of systemic administration during antigen sensitization of 
      either syngeneic or allogeneic BMSC on airways hyperreactivity, lung 
      inflammation, antigen-specific CD4 T lymphocytes, and serum immunoglobulins were 
      assessed. Both syngeneic and allogeneic BMSCs inhibited airways hyperreactivity 
      and lung inflammation through a mechanism partly dependent on IFNgamma. However, 
      contrary to existing data, BMSCs did not affect antigen-specific CD4 T lymphocyte 
      proliferation but rather promoted Th1 phenotype in vivo as assessed by both 
      OVA-specific CD4 T lymphocyte cytokine production and OVA-specific circulating 
      immunoglobulins. BMSCs treated to prevent release of soluble mediators and a 
      control cell population of primary dermal skin fibroblasts only partly mimicked 
      the BMSC effects and in some cases worsened inflammation. In conclusion, BMSCs 
      inhibit Th2-mediated allergic airways inflammation by influencing 
      antigen-specific CD4 T lymphocyte differentiation. Promotion of a Th1 phenotype 
      in antigen-specific CD4 T lymphocytes by BMSCs is sufficient to inhibit 
      Th2-mediated allergic airways inflammation through an IFNgamma-dependent process.
CI  - Copyright (c) 2011 AlphaMed Press.
FAU - Goodwin, Meagan
AU  - Goodwin M
AD  - Department of Medicine, University of Vermont College of Medicine, Burlington, 
      Vermont 05405, USA.
FAU - Sueblinvong, Viranuj
AU  - Sueblinvong V
FAU - Eisenhauer, Philip
AU  - Eisenhauer P
FAU - Ziats, Nicholas P
AU  - Ziats NP
FAU - LeClair, Laurie
AU  - LeClair L
FAU - Poynter, Matthew E
AU  - Poynter ME
FAU - Steele, Chad
AU  - Steele C
FAU - Rincon, Mercedes
AU  - Rincon M
FAU - Weiss, Daniel J
AU  - Weiss DJ
LA  - eng
GR  - T32 HL076122/HL/NHLBI NIH HHS/United States
GR  - P20 RR-155557/RR/NCRR NIH HHS/United States
GR  - HL081289/HL/NHLBI NIH HHS/United States
GR  - R21 HL081289/HL/NHLBI NIH HHS/United States
GR  - R21 HL087274/HL/NHLBI NIH HHS/United States
GR  - HL087274/HL/NHLBI NIH HHS/United States
GR  - T32 HL76122/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/immunology
MH  - Disease Models, Animal
MH  - Epitopes, T-Lymphocyte/immunology
MH  - Female
MH  - Inflammation/immunology
MH  - Interferon-gamma/immunology
MH  - Male
MH  - Mesenchymal Stem Cells/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Ovalbumin/immunology
MH  - Respiratory Hypersensitivity/*immunology
MH  - Th2 Cells/*immunology
PMC - PMC4201366
MID - NIHMS392305
EDAT- 2011/05/06 06:00
MHDA- 2011/11/16 06:00
PMCR- 2014/10/17
CRDT- 2011/05/06 06:00
PHST- 2011/05/06 06:00 [entrez]
PHST- 2011/05/06 06:00 [pubmed]
PHST- 2011/11/16 06:00 [medline]
PHST- 2014/10/17 00:00 [pmc-release]
AID - 10.1002/stem.656 [doi]
PST - ppublish
SO  - Stem Cells. 2011 Jul;29(7):1137-48. doi: 10.1002/stem.656.