PMID- 21545229
OWN - NLM
STAT- MEDLINE
DCOM- 20110818
LR  - 20170308
IS  - 2476-762X (Electronic)
IS  - 1513-7368 (Linking)
VI  - 12
IP  - 2
DP  - 2011
TI  - Monocyte chemoattractant protein-1 modulates invasion and apoptosis of PC-3M 
      prostate cancer cells via regulating expression of VEGF, MMP9 and caspase-3.
PG  - 555-9
AB  - Prostate cancer is a form of malignancy that is most likely to develop in older 
      males, but because of the propensity to metastasize to parts of the body, 
      particularly the bones, can have a deleterious impact on quality of life. 
      Recently monocyte chemoattractant protein-1 (MCP-1) has been shown to play 
      important role in prostate cancer progression and metastasi. In this study we 
      aimed to investigate the mechanisms underlying its functional roles. In vitro 
      transwell invasion assays with PC-3M prostate cancer cells demonstrated MCP-1 
      promotion of invasion, while annexin V-FITC and TUNEL confirmed inhibition of 
      apoptosis. Treatment MCP-1 further led to significant upregulation of VEGF and 
      MMP-9 and downregulation of Caspase-3 at both mRNA and protein levels compared 
      with untreated control (P < 0.05), while siRNA mediated knockdown reversed these 
      changes. Taken together, our results indicate important roles of MCP-1 in 
      prostate cancer progression and metastasis and our finding of regulation of VEGF, 
      MMP-9 and Caspase-3 expression open up new possibilities for targeted therapy.
FAU - Shi, Chao-Ling
AU  - Shi CL
AD  - Fourth Hospital of Jilin University, Changchun, China.
FAU - Yu, Chang-Hai
AU  - Yu CH
FAU - Zhang, Yu
AU  - Zhang Y
FAU - Zhao, Dan
AU  - Zhao D
FAU - Chang, Xi-Hua
AU  - Chang XH
FAU - Wang, Wei-Hua
AU  - Wang WH
LA  - eng
PT  - Journal Article
PL  - Thailand
TA  - Asian Pac J Cancer Prev
JT  - Asian Pacific journal of cancer prevention : APJCP
JID - 101130625
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - *Apoptosis
MH  - Blotting, Western
MH  - Caspase 3/genetics/*metabolism
MH  - *Cell Movement
MH  - Cell Proliferation
MH  - Chemokine CCL2/antagonists & inhibitors/genetics/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Matrix Metalloproteinase 9/genetics/*metabolism
MH  - Neoplasm Invasiveness
MH  - Prostatic Neoplasms/*metabolism/*pathology
MH  - RNA, Messenger/genetics
MH  - RNA, Small Interfering/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
MH  - Vascular Endothelial Growth Factor A/genetics/*metabolism
EDAT- 2011/05/07 06:00
MHDA- 2011/08/19 06:00
CRDT- 2011/05/07 06:00
PHST- 2011/05/07 06:00 [entrez]
PHST- 2011/05/07 06:00 [pubmed]
PHST- 2011/08/19 06:00 [medline]
PST - ppublish
SO  - Asian Pac J Cancer Prev. 2011;12(2):555-9.