PMID- 2154524
OWN - NLM
STAT- MEDLINE
DCOM- 19900326
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 47
IP  - 2
DP  - 1990 Feb
TI  - Recombinant human interleukin-1 beta primes human polymorphonuclear leukocytes 
      for stimulus-induced myeloperoxidase release.
PG  - 158-63
AB  - Recombinant human Interleukin-1 (rhIL-1) beta was found to enhance 
      stimulus-induced granule exocytosis from human polymorphonuclear leukocytes 
      (PMNs). PMNs were incubated with rhIL-1 beta and then stimulated with either 
      heat-aggregated IgG (Hagg) or N-formyl-methionyl leucylphenylalanine (FMLP). The 
      release of the azurophil enzyme myeloperoxidase (MPO) was measured. Low 
      concentrations of stimuli (10 micrograms/ml Hagg, 2.5 X 10(-9) M FMLP) did not 
      stimulate degranulation in the absence of rhIL-1 beta. However, such 
      concentrations elicited marked degranulation from PMNs preincubated with rhIL-1 
      beta (0.2-100 ng/ml). The enhancement of degranulation was dependent on the 
      concentration of rhIL-1 beta employed and on the period of incubation. In other 
      experiments, the effect of rhIL-1 beta on the PMN oxidative response was 
      determined. rhIL-1 beta did not directly stimulate the production of superoxide 
      anions or enhance the oxidative response to Hagg or FMLP. It is suggested that in 
      rheumatoid joints, IL-1 beta may potentiate PMN degranulation, but not their 
      oxidative response.
FAU - Dularay, B
AU  - Dularay B
AD  - Medical School, University of Bristol, England.
FAU - Elson, C J
AU  - Elson CJ
FAU - Clements-Jewery, S
AU  - Clements-Jewery S
FAU - Damais, C
AU  - Damais C
FAU - Lando, D
AU  - Lando D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Interleukin-1)
RN  - 0 (Recombinant Proteins)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Cell Degranulation/drug effects
MH  - Humans
MH  - Interleukin-1/*pharmacology
MH  - Neutrophils/drug effects/*enzymology
MH  - Oxidation-Reduction/drug effects
MH  - Peroxidase/*metabolism
MH  - Recombinant Proteins/*pharmacology
EDAT- 1990/02/01 00:00
MHDA- 1990/02/01 00:01
CRDT- 1990/02/01 00:00
PHST- 1990/02/01 00:00 [pubmed]
PHST- 1990/02/01 00:01 [medline]
PHST- 1990/02/01 00:00 [entrez]
AID - 10.1002/jlb.47.2.158 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1990 Feb;47(2):158-63. doi: 10.1002/jlb.47.2.158.