PMID- 21545734
OWN - NLM
STAT- MEDLINE
DCOM- 20110926
LR  - 20220318
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 12
DP  - 2011 May 5
TI  - Characterization of age-related gene expression profiling in bone marrow and 
      epididymal adipocytes.
PG  - 212
LID - 10.1186/1471-2164-12-212 [doi]
AB  - BACKGROUND: While an increase in bone marrow adiposity is associated with 
      age-related bone disease, the function of bone marrow adipocytes has not been 
      studied. The aim of this study was to characterize and compare the age-related 
      gene expression profiles in bone marrow adipocytes and epididymal adipocytes. 
      RESULTS: A total of 3918 (13.7%) genes were differentially expressed in bone 
      marrow adipocytes compared to epididymal adipocytes. Bone marrow adipocytes 
      revealed a distinct gene profile with low expression of adipocyte-specific genes 
      peroxisome proliferator-activated receptor gamma (PPARgamma), fatty acid binding 
      protein 4 (FABP4), perilipin (Plin1), adipsin (CFD) and high expression of genes 
      associated with early adipocyte differentiation (CCAAT/enhancer binding protein 
      beta (C/EBPbeta), regulator of G-protein signaling 2 (RGS2). In addition, a number 
      of genes including secreted frizzled related protein 4 (SFRP4), tumor necrosis 
      factor alpha (TNFalpha), transforming growth factor beta 1(TGFbeta1), G-protein coupled 
      receptor 109A (GPR109A) and interleukin 6 (IL-6), that could affect 
      adipose-derived signaling to bone are markedly increased in bone marrow 
      adipocytes. Age had a substantial effect on genes associated with mitochondria 
      function and inflammation in bone marrow adipocytes. Twenty seven genes were 
      significantly changed with age in both adipocyte depots. Among these genes, IL6 
      and GPR109A were significantly reduced with age in both adipocyte depots. 
      CONCLUSIONS: Overall, gene profiling reveals a unique phenotype for primary bone 
      marrow adipocytes characterized by low adipose-specific gene expression and high 
      expression of inflammatory response genes. Bone marrow and epididymal adipocytes 
      share a common pathway in response to aging in mice, but age has a greater impact 
      on global gene expression in epididymal than in bone marrow adipocytes. Genes 
      that are differentially expressed at greater levels in the bone marrow are highly 
      regulated with age.
FAU - Liu, Li-Fen
AU  - Liu LF
AD  - Division of Endocrinology, Stanford University, CA 94305-5103, USA.
FAU - Shen, Wen-Jun
AU  - Shen WJ
FAU - Ueno, Masami
AU  - Ueno M
FAU - Patel, Shailja
AU  - Patel S
FAU - Kraemer, Fredric B
AU  - Kraemer FB
LA  - eng
GR  - R01 AG028098/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20110505
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
SB  - IM
MH  - Adipocytes/*metabolism
MH  - Adipocytes, White/metabolism
MH  - Aging/*genetics
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - Epididymis/cytology
MH  - *Gene Expression Profiling
MH  - Inflammation/genetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Organ Specificity
PMC - PMC3113784
EDAT- 2011/05/07 06:00
MHDA- 2011/09/29 06:00
PMCR- 2011/05/05
CRDT- 2011/05/07 06:00
PHST- 2011/02/09 00:00 [received]
PHST- 2011/05/05 00:00 [accepted]
PHST- 2011/05/07 06:00 [entrez]
PHST- 2011/05/07 06:00 [pubmed]
PHST- 2011/09/29 06:00 [medline]
PHST- 2011/05/05 00:00 [pmc-release]
AID - 1471-2164-12-212 [pii]
AID - 10.1186/1471-2164-12-212 [doi]
PST - epublish
SO  - BMC Genomics. 2011 May 5;12:212. doi: 10.1186/1471-2164-12-212.