PMID- 21550737
OWN - NLM
STAT- MEDLINE
DCOM- 20111014
LR  - 20110613
IS  - 1873-2232 (Electronic)
IS  - 0378-4320 (Linking)
VI  - 125
IP  - 1-4
DP  - 2011 May
TI  - Risk assessment of porcine reproductive and respiratory syndrome virus (PRRSV) 
      transmission via somatic cell nuclear transfer (SCNT) embryo production using 
      oocytes from commercial abattoirs.
PG  - 148-57
LID - 10.1016/j.anireprosci.2011.04.004 [doi]
AB  - Somatic cell nuclear transfer (SCNT) technology has become a powerful tool for 
      reproductive biology to preserve and propagate valuable genetics for livestock. 
      Embryo production through SCNT involves enucleation of the oocyte and insertion 
      of a somatic donor cell into the oocyte. These procedures lead to a few small 
      openings on the zona pellucida that may elevate risk of viral infection for the 
      produced SCNT embryos. The oocytes used for SCNT are mainly obtained from 
      abattoirs where viral contamination is almost inevitable. Therefore, a systematic 
      evaluation of risk of disease transmission through SCNT embryo production is 
      necessary prior large scale implementation of this technology in the livestock 
      industry. The objective of the current study was to evaluate the risk of disease 
      transmission via SCNT embryo production and transfer by testing for the presence 
      of porcine reproductive and respiratory syndrome virus (PRRSV) throughout the 
      process of SCNT embryo production. The presence of PRRSV in each step of SCNT 
      embryo production, from donor cells to pre-implantation SCNT embryo culture, was 
      carefully examined using a real-time PCR assay with a sensitivity of five copies 
      per-reaction. All 114 donor cell lines derived from pig skin tissue over a period 
      of 7 years in our facility tested negative for PRRSV. Out of the 68 pooled 
      follicular fluid samples collected from 736 ovaries, only four (5.9%) were 
      positive indicating a small amount of viral molecule present in the oocyte donor 
      population. All 801 Day 7 SCNT embryos produced in four separate trials and over 
      11,571 washed oocytes obtained in 67 batches over 10 months tested negative. 
      These oocytes were collected from multiple abattoirs processing animals from 
      areas with high density of pig population and correspond to a donor population of 
      over 5828 individuals. These results indicate that the oocytes from abattoirs 
      were free of PRRSV infection and therefore could be safely used for in vitro 
      embryo production. Additionally, the established SCNT embryo production system, 
      including donor cell testing, oocytes decontamination, and pathogen free embryo 
      reconstruction and culturing, bears no risk of PRRSV transmission.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Gregg, K
AU  - Gregg K
AD  - Viagen, Inc., 12357-A Riata Trace Parkway, Austin, TX 78727, USA. 
      Keqin.gregg@gmail.com
FAU - Xiang, T
AU  - Xiang T
FAU - Arenivas, S S
AU  - Arenivas SS
FAU - Hwang, E
AU  - Hwang E
FAU - Arenivas, F
AU  - Arenivas F
FAU - Chen, S-H
AU  - Chen SH
FAU - Walker, S
AU  - Walker S
FAU - Picou, A
AU  - Picou A
FAU - Polejaeva, I
AU  - Polejaeva I
LA  - eng
PT  - Journal Article
DEP - 20110421
PL  - Netherlands
TA  - Anim Reprod Sci
JT  - Animal reproduction science
JID - 7807205
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Female
MH  - Follicular Fluid/virology
MH  - Nuclear Transfer Techniques/*veterinary
MH  - Oocytes/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/*transmission
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & 
      purification
MH  - RNA/chemistry/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Risk Assessment
MH  - Sensitivity and Specificity
MH  - Swine
EDAT- 2011/05/10 06:00
MHDA- 2011/10/15 06:00
CRDT- 2011/05/10 06:00
PHST- 2010/10/22 00:00 [received]
PHST- 2011/03/29 00:00 [revised]
PHST- 2011/04/14 00:00 [accepted]
PHST- 2011/05/10 06:00 [entrez]
PHST- 2011/05/10 06:00 [pubmed]
PHST- 2011/10/15 06:00 [medline]
AID - S0378-4320(11)00110-2 [pii]
AID - 10.1016/j.anireprosci.2011.04.004 [doi]
PST - ppublish
SO  - Anim Reprod Sci. 2011 May;125(1-4):148-57. doi: 
      10.1016/j.anireprosci.2011.04.004. Epub 2011 Apr 21.