PMID- 21551448
OWN - NLM
STAT- MEDLINE
DCOM- 20110817
LR  - 20110509
IS  - 1939-8697 (Electronic)
IS  - 0006-3185 (Linking)
VI  - 220
IP  - 2
DP  - 2011 Apr
TI  - Phylogenetic characterization of episymbiotic bacteria hosted by a hydrothermal 
      vent limpet (lepetodrilidae, vetigastropoda).
PG  - 118-27
AB  - Marine invertebrates hosting chemosynthetic bacterial symbionts are known from 
      multiple phyla and represent remarkable diversity in form and function. The 
      deep-sea hydrothermal vent limpet Lepetodrilus fucensis from the Juan de Fuca 
      Ridge complex hosts a gill symbiosis of particular interest because it displays a 
      morphology unique among molluscs: filamentous bacteria are found partially 
      embedded in the host's gill epithelium and extend into the fluids circulating 
      across the lamellae. Our objective was to investigate the phylogenetic 
      affiliation of the limpet's primary gill symbionts for comparison with previously 
      characterized bacteria. Comparative 16S rRNA sequence analysis identified one gamma- 
      and three epsilon-Proteobacteria as candidate symbionts. We used fluorescence in situ 
      hybridization (FISH) to test which of these four candidates occur with the 
      limpet's symbiotic gill bacteria. The gamma-proteobacterial probes consistently 
      hybridized to the entire area where symbiotic bacteria were found, but 
      fluorescence signal from the epsilon-proteobacterial probes was generally absent. 
      Amplification of the gamma-proteobacterial 16S rRNA gene using a specific forward 
      primer yielded a sequence similar to that of limpets collected from different 
      ridge sections. In total, direct amplification or FISH identified a single 
      gamma-proteobacterial lineage from the gills of 23 specimens from vents separated by 
      a distance up to about 200 km and collected over the course of 2 years, 
      suggesting a highly specific and widespread symbiosis. Thus, we report the first 
      filamentous gamma-proteobacterial gill symbiont hosted by a mollusc.
FAU - Bates, Amanda E
AU  - Bates AE
AD  - Department of Biology, University of Victoria, British Columbia, Canada. 
      amanda.bates@deakin.edu.au
FAU - Harmer, Tara L
AU  - Harmer TL
FAU - Roeselers, Guus
AU  - Roeselers G
FAU - Cavanaugh, Colleen M
AU  - Cavanaugh CM
LA  - eng
SI  - GENBANK/GQ119472
SI  - GENBANK/GQ119473
SI  - GENBANK/GQ119474
SI  - GENBANK/GQ119475
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Bull
JT  - The Biological bulletin
JID - 2984727R
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacteria/*classification/genetics/*isolation & purification
MH  - Bacterial Physiological Phenomena
MH  - *Biodiversity
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Gastropoda/*microbiology
MH  - Gills/microbiology
MH  - Hot Springs
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - *Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Symbiosis
EDAT- 2011/05/10 06:00
MHDA- 2011/08/19 06:00
CRDT- 2011/05/10 06:00
PHST- 2011/05/10 06:00 [entrez]
PHST- 2011/05/10 06:00 [pubmed]
PHST- 2011/08/19 06:00 [medline]
AID - 220/2/118 [pii]
AID - 10.1086/BBLv220n2p118 [doi]
PST - ppublish
SO  - Biol Bull. 2011 Apr;220(2):118-27. doi: 10.1086/BBLv220n2p118.