PMID- 21552960
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20190606
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 7
IP  - 6
DP  - 1995 Dec
TI  - Expression of mucinous ovarian-cancer antigen in hybrid-cells derived by fusing a 
      malignantly transformed bloom-syndrome cell-line (bs-shi-4m ovc-mu) and mouse 
      L-cell-line.
PG  - 1275-8
AB  - The expression of mucinous ovarian cancer (OVC) antigen by hybrid cell lines 
      derived by fusing a malignantly transformed Bloom syndrome (BS) cell line 
      (BS-SHI-4M OVC-MU) and mouse L cells has been studied. Cell surfaces of 16 
      hybrids which grew 12-40 days post-fusion have been analysed by using a number of 
      sera from mucinous OVC patients and indirect membrane immunofluorescence (IF). 
      Three hybrids which appeared at a late stage (30-40 days in 24-well culture 
      plates) were clearly positive in almost 100% of the hybrid cell population, while 
      all of those which appeared earlier (in 12 days) were completely negative. 
      Cytogenetic analysis showed that these three hybrids with positive mucinous OVC 
      antigen contained human chromosome 22 or 22q-, though no metaphases from antigen 
      negative population had 22 or 22q-. Therefore, we assign the gene for mucinous 
      OVC antigen to the chromosome 22. Identification of chromosome 22 in hybrid cells 
      was confirmed by the fluorescence in situ hybridization (FISH) analysis using 
      chromosome 22 painting probe.
FAU - Shiraishi, Y
AU  - Shiraishi Y
AD  - KOCHI MED SCH,DEPT ANAT 2,NANKOKU,KOCHI 783,JAPAN.
FAU - Taguchi, T
AU  - Taguchi T
FAU - Seguchi, H
AU  - Seguchi H
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 2011/05/10 06:00
PHST- 2011/05/10 06:00 [entrez]
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
AID - 10.3892/ijo.7.6.1275 [doi]
PST - ppublish
SO  - Int J Oncol. 1995 Dec;7(6):1275-8. doi: 10.3892/ijo.7.6.1275.