PMID- 21553221
OWN - NLM
STAT- MEDLINE
DCOM- 20120117
LR  - 20211020
IS  - 1432-1777 (Electronic)
IS  - 0938-8990 (Linking)
VI  - 22
IP  - 9-10
DP  - 2011 Oct
TI  - Generation of N-ethyl-N-nitrosourea-induced mouse mutants with deviations in 
      hematological parameters.
PG  - 495-505
LID - 10.1007/s00335-011-9328-4 [doi]
AB  - Research on hematological disorders relies on suitable animal models. We 
      retrospectively evaluated the use of the hematological parameters hematocrit 
      (HCT), hemoglobin (HGB), mean corpuscular hemoglobin (MCH), mean corpuscular 
      hemoglobin concentration (MCHC), mean corpuscular volume (MCV), red blood cell 
      count (RBC), white blood cell count (WBC), and platelet count (PLT) in the 
      phenotype-driven Munich N-ethyl-N-nitrosourea (ENU) mouse mutagenesis project as 
      parameters for the generation of novel animal models for human diseases. The 
      analysis was carried out on more than 16,000 G1 and G3 offspring of chemically 
      mutagenized inbred C3H mice to detect dominant and recessive mutations leading to 
      deviations in the levels of the chosen parameters. Identification of animals 
      exhibiting altered values and transmission of the phenotypic deviations to the 
      subsequent generations led to the successful establishment of mutant lines for 
      the parameters MCV, RBC, and PLT. Analysis of the causative mutation was started 
      in selected lines, thereby revealing a novel mutation in the transferrin receptor 
      gene (Tfrc) in one line. Thus, novel phenotype-driven mouse models were 
      established to analyze the genetic components of hematological disorders.
FAU - Aigner, Bernhard
AU  - Aigner B
AD  - Department of Veterinary Sciences, Chair for Molecular Animal Breeding and 
      Biotechnology, and Laboratory for Functional Genome Analysis (LAFUGA), Gene 
      Center, LMU Munich, Moorversuchsgut, Hackerstr. 27, 85764, Oberschleibetaheim, 
      Munich, Germany. b.aigner@gen.vetmed.uni-muenchen.de
FAU - Rathkolb, Birgit
AU  - Rathkolb B
FAU - Klempt, Martina
AU  - Klempt M
FAU - Wagner, Sibylle
AU  - Wagner S
FAU - Michel, Dian
AU  - Michel D
FAU - Klaften, Matthias
AU  - Klaften M
FAU - Laufs, Jurgen
AU  - Laufs J
FAU - Schneider, Boris
AU  - Schneider B
FAU - Sedlmeier, Reinhard
AU  - Sedlmeier R
FAU - Hrabe de Angelis, Martin
AU  - Hrabe de Angelis M
FAU - Wolf, Eckhard
AU  - Wolf E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110508
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (Mutagens)
RN  - 0 (Receptors, Transferrin)
RN  - 0 (Tfrc protein, mouse)
RN  - P8M1T4190R (Ethylnitrosourea)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - *Disease Models, Animal
MH  - Ethylnitrosourea
MH  - Female
MH  - Genetic Linkage
MH  - Genotype
MH  - Hematologic Diseases/*genetics
MH  - Hematologic Tests
MH  - Male
MH  - Mice/*genetics
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - *Mutagenesis
MH  - Mutagens
MH  - *Mutation
MH  - Phenotype
MH  - Receptors, Transferrin/genetics
MH  - Reference Values
EDAT- 2011/05/10 06:00
MHDA- 2012/01/18 06:00
CRDT- 2011/05/10 06:00
PHST- 2011/02/01 00:00 [received]
PHST- 2011/04/14 00:00 [accepted]
PHST- 2011/05/10 06:00 [entrez]
PHST- 2011/05/10 06:00 [pubmed]
PHST- 2012/01/18 06:00 [medline]
AID - 10.1007/s00335-011-9328-4 [doi]
PST - ppublish
SO  - Mamm Genome. 2011 Oct;22(9-10):495-505. doi: 10.1007/s00335-011-9328-4. Epub 2011 
      May 8.