PMID- 21553849
OWN - NLM
STAT- MEDLINE
DCOM- 20111201
LR  - 20220410
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 83
IP  - 11
DP  - 2011 Jun 1
TI  - Infrared and Raman spectroscopic studies of the antimicrobial effects of garlic 
      concentrates and diallyl constituents on foodborne pathogens.
PG  - 4137-46
LID - 10.1021/ac2001498 [doi]
AB  - The antimicrobial effects of garlic (Allium sativum) extract (25, 50, 75, 100, 
      and 200 muL/ml) and diallyl sulfide (5, 10, and 20 muM) on Listeria monocytogenes 
      and Escherichia coli O157:H7 cultivated in tryptic soy broth at 4, 22, and 35  degrees C 
      for up to 7 days were investigated. L. monocytogenes was more resistant to garlic 
      extract and diallyl compounds treatment than E. coli O157:H7. Fourier transform 
      infrared (FT-IR) spectroscopy indicated that diallyl constituents contributed 
      more to the antimicrobial effect than phenolic compounds. This effect was 
      verified by Raman spectroscopy and Raman mapping on single bacteria. Scanning 
      electron microscope (SEM) and transmission electron microscope (TEM) showed cell 
      membrane damage consistent with spectroscopic observation. The degree of 
      bacterial cell injury could be quantified using chemometric methods.
FAU - Lu, Xiaonan
AU  - Lu X
AD  - School of Food Science, Washington State University, Pullman 99163, USA.
FAU - Rasco, Barbara A
AU  - Rasco BA
FAU - Kang, Dong Hyun
AU  - Kang DH
FAU - Jabal, Jamie M F
AU  - Jabal JM
FAU - Aston, D Eric
AU  - Aston DE
FAU - Konkel, Michael E
AU  - Konkel ME
LA  - eng
GR  - R56 AI088518/AI/NIAID NIH HHS/United States
GR  - N01-AI-30055/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20110509
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Allyl Compounds)
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Phenols)
RN  - 0 (Plant Extracts)
RN  - 0 (Sulfides)
RN  - 60G7CF7CWZ (allyl sulfide)
SB  - IM
MH  - Allyl Compounds/*pharmacology
MH  - Anti-Infective Agents/*pharmacology
MH  - Escherichia coli O157/drug effects
MH  - Food Microbiology
MH  - Garlic/*chemistry
MH  - Listeria monocytogenes/drug effects
MH  - Microscopy, Electron, Transmission
MH  - Phenols/pharmacology
MH  - Plant Extracts/*pharmacology
MH  - Spectroscopy, Fourier Transform Infrared/*methods
MH  - Spectrum Analysis, Raman/*methods
MH  - Sulfides/*pharmacology
PMC - PMC3433400
MID - NIHMS295655
EDAT- 2011/05/11 06:00
MHDA- 2011/12/13 00:00
PMCR- 2012/09/04
CRDT- 2011/05/11 06:00
PHST- 2011/05/11 06:00 [entrez]
PHST- 2011/05/11 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2012/09/04 00:00 [pmc-release]
AID - 10.1021/ac2001498 [doi]
PST - ppublish
SO  - Anal Chem. 2011 Jun 1;83(11):4137-46. doi: 10.1021/ac2001498. Epub 2011 May 9.