PMID- 21554192
OWN - NLM
STAT- MEDLINE
DCOM- 20120111
LR  - 20220309
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Print)
IS  - 1937-3341 (Linking)
VI  - 17
IP  - 19-20
DP  - 2011 Oct
TI  - The effect of mesenchymal stromal cell-hyaluronic acid hydrogel constructs on 
      immunophenotype of macrophages.
PG  - 2463-71
LID - 10.1089/ten.TEA.2010.0716 [doi]
AB  - During the past several years, multipotent mesenchymal stromal cells (MSCs) have 
      rapidly moved from in vitro and animal studies into clinical trials as a 
      therapeutic modality potentially applicable to a wide range of disorders. It has 
      been proposed that ex vivo culture-expanded MSCs exert their tissue regeneration 
      potential through their immunomodulatory and anti-inflammatory properties, and 
      paracrine effects more than their ability to differentiate into multiple tissue 
      lineages. Since extracellular matrix (ECM) deposition and tissue support is also 
      one of many physiological roles of MSCs, there is increasing interest in their 
      potential use for tissue engineering, particularly in combination with ECM-based 
      scaffolds such as hyaluronic acid (HA). We investigated the effect of MSCs on 
      immunophenotype of macrophages in the presence of an HA-hydrogel scaffold using a 
      unique 3D coculture system. MSCs were encapsulated in the hydrogel and peripheral 
      blood CD14+ monocyte-derived macrophages plated in direct contact with the 
      MSC-gel construct. To determine the immunophenotype of macrophages, we looked at 
      the expression of cell surface markers CD14, CD16, CD206, and human leukocyte 
      antigen (HLA)-DR by flow cytometry. MSCs and macrophages cultured on the 
      HA-hydrogel remained viable and were able to be recovered from the construct. 
      There was a significant difference in the immunophenotype observed between 
      monocyte-derived macrophages cultured on the HA scaffold compared to tissue 
      culture polystyrene. Macrophages cultured on gels with MSCs expressed lower CD16 
      and HLA-DR with higher expression of CD206, indicating the least inflammatory 
      profile overall, compatible with the immunophenotype of alternatively activated 
      macrophages. Development of macrophages, with this immunophenotype, upon 
      interaction with the MSC-hydrogel constructs may play a potentially significant 
      role in tissue repair when using a cellular-biomaterial therapeutic approach.
CI  - (c) Mary Ann Liebert, Inc.
FAU - Hanson, Summer E
AU  - Hanson SE
AD  - Division of Plastic and Reconstructive Surgery, University of Wisconsin-Madison, 
      School of Medicine and Public Health, Madison, Wisconsin 53705, USA.
FAU - King, Suzanne N
AU  - King SN
FAU - Kim, Jaehyup
AU  - Kim J
FAU - Chen, Xia
AU  - Chen X
FAU - Thibeault, Susan L
AU  - Thibeault SL
FAU - Hematti, Peiman
AU  - Hematti P
LA  - eng
GR  - T32 DC009401/DC/NIDCD NIH HHS/United States
GR  - R01 DC004336/DC/NIDCD NIH HHS/United States
GR  - R01 DC4336/DC/NIDCD NIH HHS/United States
GR  - T32 CA009614/CA/NCI NIH HHS/United States
GR  - K08 HL081076/HL/NHLBI NIH HHS/United States
GR  - R01 DC4336 S1/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20110624
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Mannose Receptor)
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, IgG)
RN  - 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate)
RN  - 9004-61-9 (Hyaluronic Acid)
SB  - IM
MH  - Cell Membrane/metabolism
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Flow Cytometry
MH  - HLA-DR Antigens/immunology
MH  - Humans
MH  - Hyaluronic Acid/*pharmacology
MH  - Hydrogel, Polyethylene Glycol Dimethacrylate/*pharmacology
MH  - Immunophenotyping/*methods
MH  - Lectins, C-Type/metabolism
MH  - Lipopolysaccharide Receptors/metabolism
MH  - Macrophages/cytology/drug effects/*immunology
MH  - Mannose Receptor
MH  - Mannose-Binding Lectins/metabolism
MH  - Mesenchymal Stem Cells/*cytology/drug effects/metabolism
MH  - Monocytes/cytology/drug effects
MH  - Receptors, Cell Surface/metabolism
MH  - Receptors, IgG/metabolism
MH  - Tissue Scaffolds/*chemistry
PMC - PMC3179618
EDAT- 2011/05/11 06:00
MHDA- 2012/01/12 06:00
PMCR- 2012/10/01
CRDT- 2011/05/11 06:00
PHST- 2011/05/11 06:00 [entrez]
PHST- 2011/05/11 06:00 [pubmed]
PHST- 2012/01/12 06:00 [medline]
PHST- 2012/10/01 00:00 [pmc-release]
AID - 10.1089/ten.tea.2010.0716 [pii]
AID - 10.1089/ten.TEA.2010.0716 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2011 Oct;17(19-20):2463-71. doi: 10.1089/ten.TEA.2010.0716. 
      Epub 2011 Jun 24.