PMID- 2155478
OWN - NLM
STAT- MEDLINE
DCOM- 19900404
LR  - 20191022
IS  - 0740-7750 (Print)
IS  - 0740-7750 (Linking)
VI  - 16
IP  - 1
DP  - 1990 Jan
TI  - Gene regulation in reverse transformation: cyclic AMP-induced actin homolog in 
      CHO cells.
PG  - 67-78
AB  - Reverse transformation (RT) presents a challenge in understanding of the role of 
      protein-genome interaction in regulating gene expression in normal and 
      transformed cells. Early during RT of CHO-K1 cells by cyclic AMP a new protein, 
      mol wt = 43,000 and pI = 5.3 +/- 0.2, was rapidly and specifically induced. This 
      cAMP-induced protein (CIP) is a phosphorylated actin homolog. Induction required 
      new protein synthesis. Actinomycin D treatment failed to inhibit CIP induction, 
      suggesting the existence of an untranslated or sequestered mRNA in untreated 
      cells. Expression of CIP was not dependent upon cell shape or cytoskeletal 
      integrity as are other steps in RT. CIP was detectable only in cAMP-treated 
      cells, whether transformed or nontransformed, and cAMP treatment inhibited growth 
      of both cell types. CIP was associated with soluble cell fractions and not with 
      F-actin. We propose that CIP plays an early role in RT, that is necessary but not 
      sufficient for the complete RT process, and that it participates in the cAMP 
      signaling pathway of cells through changes in the cytoskeleton. This pathway 
      inhibits cell growth as required in the differentiated phenotype. A molecular 
      model is presented for the RT reaction in CHO-K1, which also explains cAMP 
      effects on transformed cells such as the S49 lymphoma and other malignancies.
FAU - Miranti, C
AU  - Miranti C
AD  - Eleanor Roosevelt Institute for Cancer Research, Denver, Colorado.
FAU - Puck, T T
AU  - Puck TT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Somat Cell Mol Genet
JT  - Somatic cell and molecular genetics
JID - 8403568
RN  - 0 (Actins)
RN  - 0 (Phosphoproteins)
RN  - E0399OZS9N (Cyclic AMP)
SB  - IM
MH  - Actins/genetics
MH  - Animals
MH  - Blotting, Western
MH  - Cell Fractionation
MH  - Cell Line
MH  - Cell Transformation, Neoplastic/*genetics
MH  - Cricetinae
MH  - Cyclic AMP/*physiology
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Gene Expression Regulation
MH  - Phosphoproteins/*biosynthesis/genetics
MH  - Phosphorylation
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1007/BF01650481 [doi]
PST - ppublish
SO  - Somat Cell Mol Genet. 1990 Jan;16(1):67-78. doi: 10.1007/BF01650481.