PMID- 21556766
OWN - NLM
STAT- MEDLINE
DCOM- 20120321
LR  - 20211020
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 39
IP  - 1
DP  - 2012 Jan
TI  - The 156KELK159 tetrapeptide of HIV-1 integrase is critical for lentiviral gene 
      integration.
PG  - 343-9
LID - 10.1007/s11033-011-0744-z [doi]
AB  - HIV-1 integrase (HIV-1 IN), a key element of HIV-1-derived lentiviral vectors, is 
      crucial for the stable maintenance of the vector gene by inserting them into host 
      genome. HIV-1 IN has been found to have functions other than integration, such as 
      involving in virion morphology, viral DNA synthesis and viral DNA nuclear import. 
      In our study, the yeast two-hybrid assay identified a tetrapeptide 156KELK159 in 
      HIV-1 IN that was crucial for HIV-1 IN and Daxx interaction. To investigate the 
      functions of the tetrapeptide 156KELK159 of the HIV-1 IN, both the wild type 
      HIV-1 IN and a mutant without 156KELK159 were used to package the EGFP reporter 
      gene contained lentivirus. p24 based titer assay revealed that deleting the 
      tetrapeptide did not affect virus packaging. The result was verified by 
      quantitative real time PCR with viral specific primers. But the 156KELK159 was 
      crucial for lentiviral gene integration. Deleting the tetrapeptide made the 
      percentage of cells expressing the reporter gene significantly decreased and did 
      not affect the level of DNA entered into the cells or nucleus. Real time reverse 
      transcription PCR and FACS were used to detect the lentiviral report gene 
      expression in infection maintaining cells and revealed 156KELK159 did not affect 
      lentiviral vector gene expression. Our results may shed light on the regulatory 
      mechanism of gene integration of lentivirus.
FAU - Li, Wen-juan
AU  - Li WJ
AD  - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of 
      Life Sciences, Fudan University, Shanghai, China.
FAU - Huang, Lu
AU  - Huang L
FAU - Zhang, Jian-qi
AU  - Zhang JQ
FAU - Xu, Guan-lan
AU  - Xu GL
FAU - Tian, Ling
AU  - Tian L
FAU - Xue, Jing-lun
AU  - Xue JL
FAU - Chen, Jin-zhong
AU  - Chen JZ
FAU - Jia, William
AU  - Jia W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110510
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Co-Repressor Proteins)
RN  - 0 (DAXX protein, human)
RN  - 0 (DNA Primers)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Oligopeptides)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.7.7.- (HIV Integrase)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/metabolism
MH  - Cell Line
MH  - Co-Repressor Proteins
MH  - DNA Primers/genetics
MH  - Genetic Vectors
MH  - Green Fluorescent Proteins/metabolism
MH  - HIV Integrase/*genetics
MH  - HIV-1/*genetics
MH  - Humans
MH  - Molecular Chaperones
MH  - Nuclear Proteins/metabolism
MH  - Oligopeptides/genetics/*physiology
MH  - Plasmids/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transduction, Genetic/*methods
MH  - Two-Hybrid System Techniques
EDAT- 2011/05/11 06:00
MHDA- 2012/03/22 06:00
CRDT- 2011/05/11 06:00
PHST- 2009/01/03 00:00 [received]
PHST- 2011/04/27 00:00 [accepted]
PHST- 2011/05/11 06:00 [entrez]
PHST- 2011/05/11 06:00 [pubmed]
PHST- 2012/03/22 06:00 [medline]
AID - 10.1007/s11033-011-0744-z [doi]
PST - ppublish
SO  - Mol Biol Rep. 2012 Jan;39(1):343-9. doi: 10.1007/s11033-011-0744-z. Epub 2011 May 
      10.