PMID- 21557029
OWN - NLM
STAT- MEDLINE
DCOM- 20111115
LR  - 20211020
IS  - 1543-706X (Electronic)
IS  - 1071-2690 (Linking)
VI  - 47
IP  - 7
DP  - 2011 Aug
TI  - Effect of glucocorticoids pretreatment on steroidogenic capacity of 
      adrenocortical cells isolated from Meishan piglets.
PG  - 425-30
LID - 10.1007/s11626-011-9421-8 [doi]
AB  - The present in vitro experiment was designed to test whether 48 h of pretreatment 
      with glucocorticoids, cortisol, or dexamethasone (DEX), would affect basal and 
      corticotrophin (ACTH) stimulated (24 h) cortisol secretion from primary cultures 
      of pig adrenocortical cells. Cells were divided into six groups: control 
      pretreatment with or without ACTH challenge, cortisol pretreatment with or 
      without ACTH challenge, and DEX pretreatment with or without ACTH. The culture 
      medium and cells were collected at the end of treatment. Cortisol concentration 
      in medium was measured by radioimmunoassay, and protein content of glucocorticoid 
      receptor (GR) and key regulatory factors for steroidogenesis, including 
      melanocortin type 2 receptor (MC2R), steroidogenic acute regulatory protein 
      (StAR) and cholesterol side-chain cleavage cytochrome P450 (P450scc), were 
      detected by Western blot analysis. The results showed that glucocorticoid 
      pretreatment did not affect cortisol secretion under basal condition without ACTH 
      challenge, but significantly enhanced ACTH-stimulated cortisol secretion. 
      Furthermore, the protein content of GR, MC2R, StAR, and P450scc was all increased 
      in groups pretreated with glucocorticoids. These results indicate that 
      adrenocortical cells pretreated with glucocorticoids display higher steroidogenic 
      capacity under ACTH challenge, through the upregulation of GR and other 
      steroidogenic regulatory factors.
FAU - Li, Liu-An
AU  - Li LA
AD  - Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural 
      University, Nanjing, People's Republic of China.
FAU - Wu, Zhan-Wang
AU  - Wu ZW
FAU - Yang, Xiao-Jing
AU  - Yang XJ
FAU - Ni, Ying-Dong
AU  - Ni YD
FAU - Parvizi, Nahid
AU  - Parvizi N
FAU - Zhao, Ru-Qian
AU  - Zhao RQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110510
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
RN  - 0 (Glucocorticoids)
RN  - 0 (Phosphoproteins)
RN  - 0 (steroidogenic acute regulatory protein)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 9002-60-2 (Adrenocorticotropic Hormone)
RN  - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Adrenal Glands/cytology/*drug effects/metabolism
MH  - Adrenocorticotropic Hormone/*pharmacology
MH  - Animals
MH  - Cells, Cultured
MH  - Cholesterol Side-Chain Cleavage Enzyme/metabolism
MH  - Dexamethasone/*pharmacology
MH  - Glucocorticoids/*pharmacology
MH  - Hydrocortisone/metabolism/*pharmacology
MH  - Male
MH  - Phosphoproteins/metabolism
MH  - *Swine
EDAT- 2011/05/11 06:00
MHDA- 2011/11/16 06:00
CRDT- 2011/05/11 06:00
PHST- 2011/03/07 00:00 [received]
PHST- 2011/04/20 00:00 [accepted]
PHST- 2011/05/11 06:00 [entrez]
PHST- 2011/05/11 06:00 [pubmed]
PHST- 2011/11/16 06:00 [medline]
AID - 10.1007/s11626-011-9421-8 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 2011 Aug;47(7):425-30. doi: 
      10.1007/s11626-011-9421-8. Epub 2011 May 10.