PMID- 21557514
OWN - NLM
STAT- MEDLINE
DCOM- 20111013
LR  - 20211203
IS  - 1934-6638 (Electronic)
IS  - 1934-662X (Linking)
VI  - 119
IP  - 4
DP  - 2011 Aug 25
TI  - Noninvasive detection of aneuploid cells in laryngeal epithelial precursor 
      lesions.
PG  - 235-46
LID - 10.1002/cncy.20157 [doi]
AB  - BACKGROUND: Most cases of laryngeal cancer are preceded by precursor lesions 
      which, if left untreated, can progress toward an invasive cancer. The objective 
      of this study was to investigate the presence of chromosomal numerical 
      aberrations in cells that were collected by noninvasive brush sampling from 
      laryngeal lesions. METHODS: Laryngeal brush samples from 52 patients were 
      analyzed simultaneously for morphology and fluorescence in situ hybridization 
      (FISH) using centromeric probes for chromosome 17, chromosome 8, and a 
      locus-specific instability (LSI) v-myc avian myelocytomatosis viral oncogene 
      homolog (myc) proto-oncogene protein (C-MYC) probe for the MYC gene. The patients 
      were divided according to histopathologic diagnosis. Group 1 included patients 
      with squamous cell carcinoma, carcinoma in situ, and severe dysplasia; Group 2 
      included patients with moderate dysplasia, mild dysplasia, and hyperplasia; and 
      Group 3 included patients with benign nondysplastic lesions. RESULTS: The 
      proportion of cells with MYC and chromosome 8 gains demonstrated significant 
      trends toward being the highest in Group 1 and the lowest in Group 3 (P = .001 
      and P = .003, respectively). No significant trend was observed for chromosome 17. 
      Mann-Whitney Bonferroni-corrected analyses revealed that the most significant 
      contribution was the difference between Groups 1 and 3 (P = .0195 for MYC gains 
      and P = .036 for chromosome 8 gains). When using a cutoff point of 4% aneuploid 
      cells (ACs), both MYC and chromosome 8 differed significantly between groups (P = 
      .030 and P = .037, respectively). CONCLUSIONS: The current results suggested that 
      FISH analysis of brush samples obtained noninvasively from suspicious laryngeal 
      lesions can augment the clinical examination in predicting the nature of the 
      lesions and can aid clinicians in monitoring and follow-up of high-risk patients. 
      Cancer (Cancer Cytopathol) 2011. (c) 2011 American Cancer Society.
CI  - Copyright (c) 2011 American Cancer Society.
FAU - Shani, Tali
AU  - Shani T
AD  - Department of Oral Pathology and Oral Medicine, The Maurice and Gabriela 
      Goldschleger School of Dental Medicine, Tel Aviv University, Israel.
FAU - Primov-Fever, Adi
AU  - Primov-Fever A
FAU - Wolf, Michael
AU  - Wolf M
FAU - Shalmon, Bruria
AU  - Shalmon B
FAU - Amarglio, Ninette
AU  - Amarglio N
FAU - Trakhtenbrot, Luba
AU  - Trakhtenbrot L
FAU - Hirshberg, Abraham
AU  - Hirshberg A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110506
PL  - United States
TA  - Cancer Cytopathol
JT  - Cancer cytopathology
JID - 101499453
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Aneuploidy
MH  - Carcinoma in Situ/*diagnosis/genetics
MH  - Case-Control Studies
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - Hyperplasia/*diagnosis/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Laryngeal Neoplasms/*diagnosis/genetics
MH  - Larynx/*pathology
MH  - Male
MH  - Middle Aged
MH  - Precancerous Conditions/*diagnosis/genetics
MH  - Prognosis
MH  - Proto-Oncogene Mas
EDAT- 2011/05/11 06:00
MHDA- 2011/10/14 06:00
CRDT- 2011/05/11 06:00
PHST- 2010/12/30 00:00 [received]
PHST- 2011/03/11 00:00 [revised]
PHST- 2011/03/17 00:00 [accepted]
PHST- 2011/05/11 06:00 [entrez]
PHST- 2011/05/11 06:00 [pubmed]
PHST- 2011/10/14 06:00 [medline]
AID - 10.1002/cncy.20157 [doi]
PST - ppublish
SO  - Cancer Cytopathol. 2011 Aug 25;119(4):235-46. doi: 10.1002/cncy.20157. Epub 2011 
      May 6.