PMID- 21557685
OWN - NLM
STAT- MEDLINE
DCOM- 20120131
LR  - 20111006
IS  - 1369-1635 (Electronic)
IS  - 0953-7104 (Linking)
VI  - 22
IP  - 7
DP  - 2011
TI  - Platelet-monocyte cross talk and tissue factor expression in stable angina vs. 
      unstable angina/non ST-elevation myocardial infarction.
PG  - 530-6
LID - 10.3109/09537104.2011.573599 [doi]
AB  - Tissue factor (TF), the major procoagulant in vivo, is usually absent from blood 
      cells. However, since both monocyte TF (MoTF) expression and platelet activation 
      are present in acute coronary syndrome we hypothesized that MoTF expression may 
      in part depend on monocyte platelet aggregate (MPA) formation in coronary artery 
      disease (CAD). Patients with unstable angina/non-ST-elevation myocardial 
      infarction (UA/NSTEMI, n = 20) had significantly higher levels of MoTF 
      (17.4 +/- 3.1MFI) and MPAs (CD42b:273 +/- 183MFI; CD62P:256.3 +/- 48.5MFI) than 
      patients with stable angina (SA, n = 40; MoTF:13.2 +/- 2.2MFI, p = 0.001; 
      CD42b:160 +/- 113MFI, p = 0.025; CD62P:118.7 +/- 24.5MFI, p = 0.018) as measured by 
      whole blood flow cytometry on CD14+-cells. TF-activity of isolated mononuclear 
      cells (MNC) was elevated in UA/NSTEMI (75 +/- 27 pg/mL) in comparison to SA 
      (47 +/- 17 pg/mL, p = 0.001) as determined by chromogenic assay, and TF mRNA 
      expression in isolated MNC was more frequent in UA/NSTEMI than in SA (50% vs. 
      18.2%; p = 0.017). MoTF expression significantly correlated with the constitutive 
      platelet marker CD42b (r = 0.69, p < 0.001) and the platelet activation marker 
      CD62P (r = 0.47, p = 0.001) on CD14+-cells suggesting its association with MPAs 
      in UA/NSTEMI. In addition, MoTF expression correlated with MoTF activity of 
      isolated MNC (r = 0.41, p = 0.01) and plasma levels of the F1.2 prothrombin 
      fragment (r = 0.35, p = 0.02). In conclusion, MoTF and MPAs are elevated in 
      UA/NSTEMI compared with SA. MoTF expression correlates with platelet mass and 
      activity attached to monocytes.
FAU - Kopp, Christoph W
AU  - Kopp CW
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, Vienna, Austria. christoph.kopp@meduniwien.ac.at
FAU - Gremmel, Thomas
AU  - Gremmel T
FAU - Steiner, Sabine
AU  - Steiner S
FAU - Seidinger, Daniela
AU  - Seidinger D
FAU - Minar, Erich
AU  - Minar E
FAU - Maurer, Gerald
AU  - Maurer G
FAU - Huber, Kurt
AU  - Huber K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110511
PL  - England
TA  - Platelets
JT  - Platelets
JID - 9208117
RN  - 0 (RNA, Messenger)
RN  - 9035-58-9 (Thromboplastin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Angina, Stable/drug therapy/*metabolism
MH  - Angina, Unstable/drug therapy/*metabolism
MH  - Blood Platelets/*metabolism
MH  - *Cell Communication
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*metabolism
MH  - Myocardial Infarction/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Thromboplastin/genetics/*metabolism
EDAT- 2011/05/12 06:00
MHDA- 2012/02/01 06:00
CRDT- 2011/05/12 06:00
PHST- 2011/05/12 06:00 [entrez]
PHST- 2011/05/12 06:00 [pubmed]
PHST- 2012/02/01 06:00 [medline]
AID - 10.3109/09537104.2011.573599 [doi]
PST - ppublish
SO  - Platelets. 2011;22(7):530-6. doi: 10.3109/09537104.2011.573599. Epub 2011 May 11.